高等学校化学学报 ›› 2017, Vol. 38 ›› Issue (5): 866-871.doi: 10.7503/cjcu20170030

• 高分子化学 • 上一篇    下一篇

载万古霉素PLGA-PEG-PLGA温度敏感水凝胶的体外抗菌性能

董晓明1, 袁宝明1, 陈炳鹏1, 贺超良2, 王金成1, 陈学思2   

  1. 1. 吉林大学第二医院, 长春 130041;
    2. 中国科学院长春应用化学研究所, 中国科学院生态环境高分子材料重点实验室, 长春 130022
  • 收稿日期:2017-01-16 修回日期:2017-04-12 出版日期:2017-05-10 发布日期:2017-04-12
  • 通讯作者: 王金成,男,博士,教授,博士生导师,主要从事人工关节与组织工程研究.E-mail:jinchengwang2015@gmail.com;贺超良,男,博士,研究员,主要从事仿生高分子水凝胶的制备与生物医学应用研究.E-mail:clhe@ciac.ac.cn E-mail:jinchengwang2015@gmail.com;clhe@ciac.ac.cn
  • 基金资助:

    吉林省科技厅专利推进项目(批准号:20150312028ZG)资助.

In vitro Antibacterial Activity of PLGA-PEG-PLGA Thermosensitive Hydrogels Loaded with Vancomycin

DONG Xiaoming1, YUAN Baoming1, CHEN Bingpeng1, HE Chaoliang2, WANG Jincheng1, CHEN Xuesi2   

  1. 1. The Second Hospital, Jilin University, Changchun 130041, China;
    2. Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  • Received:2017-01-16 Revised:2017-04-12 Online:2017-05-10 Published:2017-04-12
  • Supported by:

    Supported by the Patent Transformation and Promotion Project of Jilin Science and Technology Department, China(No.20150312028ZG).

摘要:

利用聚乙二醇(PEG 1500)引发乙交酯和D,L-丙交酯开环共聚合制备聚丙交酯乙交酯(PLGA)三嵌段共聚物(PLGA-PEG-PLGA)温敏水凝胶材料,并通过核磁共振氢谱(1H NMR)确定产物的结构及组成.应用倒置小瓶法测量得到不同浓度下PLGA-PEG-PLGA水凝胶的溶胶-凝胶相变温度为27~32℃.此外,体外降解实验及细胞毒性实验结果表明,质量分数为25%的水凝胶有满意的降解速度及良好的生物相容性.同时,利用紫外-可见光谱分析了载万古霉素水凝胶的体外药物释放行为,结果表明,万古霉素可以持续释放12 d.抗菌实验结果表明,载万古霉素水凝胶具有良好的抗菌效果.表明PLGA-PEG-PLGA三嵌段温敏水凝胶是一种较理想的万古霉素缓释载体,具有良好的临床应用前景.

关键词: 聚丙交酯乙交酯与聚乙二醇的三嵌段共聚物, 温度敏感水凝胶, 万古霉素, 药物缓释, 抗菌性能

Abstract:

The poly(D,L-lactide-co-glycolide)-poly(ethylene glycol)-poly(D,L-lactide-co-glycolide)(PLGA-PEG-PLGA) triblock copolymer was synthesized via ring opening copolymerization of D,L-lactide(D,L-LA) and glycolide(GA) by PEG(Mn=1500) as a macromolecular initiator. The chemical structure and composition of the copolymer were determined by 1H NMR spectra. The sol-gel phase transitions of the PLGA-PEG-PLGA solutions in PBS(pH=7.4) were studied by the vial tilting method. The phase-transition temperatures of polymer with different concentrations were from 27℃ to 32℃, which confirmed by rheology analysis. In addition, the degradation and biocompatibility of the 25%(mass fraction) hydrogels were investigated in vitro. And the results show a satisfied degradation behavior and good cyto-compatibility. Meanwhile, UV-Vis spectroscopy was used to measure the release properties of vancomycin loaded thermosensitive hydrogel. The release of vancomycin lasted to 12 d. The result of antibacterial experiment in vitro indicated that the vancomycin loaded thermosensitive hydrogels possessed excellent antibacterial properties. Therefore, the PLGA thermosensitive hydrogels hold potential as promising platforms for delivery of vancomycin for preventing infections in clinical treatment.

Key words: PLGA-PEG-PLGA triblock copolymer, Thermosensitive hydrogel, Vancomycin, Drug sustained release, Antibacterial activity

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