高等学校化学学报 ›› 2015, Vol. 36 ›› Issue (7): 1269-1274.doi: 10.7503/cjcu20150273

• 分析化学 • 上一篇    下一篇

纳米银比色法检测多巴胺

冯娟娟, 赵祎曼, 王海燕()   

  1. 安徽师范大学化学与材料科学学院, 芜湖 241000
  • 收稿日期:2015-04-07 出版日期:2015-07-10 发布日期:2015-06-11
  • 作者简介:联系人简介: 王海燕, 女, 博士, 教授, 主要从事电分析化学研究. E-mail:hywang@mail.ahnu.edu.cn
  • 基金资助:
    国家自然科学基金(批准号: 21345004)资助

Colorimetric Detection of Dopamine Based on Silver Nanoparticles

FENG Juanjuan, ZHAO Yiman, WANG Haiyan*()   

  1. Anhui Key Laboratory of Chemo-biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, China
  • Received:2015-04-07 Online:2015-07-10 Published:2015-06-11
  • Contact: WANG Haiyan E-mail:hywang@mail.ahnu.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.21345004)

摘要:

在银纳米粒子存在下, 多巴胺可还原硝酸银生成银, 导致银纳米粒子粒径增大, 从而使溶液颜色发生改变. 基于此, 提出了一种用于检测多巴胺的纳米银比色法. 随着多巴胺浓度的增大, 溶液的颜色由浅黄色逐渐变为深黄色, 银纳米粒子溶液的吸收峰发生红移且吸光度增大. 在最优实验条件下, 该方法检测多巴胺的线性范围为0.05~16 μmol/L, 检出限为0.04 μmol/L. 该方法操作简单、 灵敏且选择性良好, 可用于人血清中多巴胺的检测.

关键词: 银纳米粒子, 比色法, 多巴胺

Abstract:

A simple and sensitive colorimetric method for the detection of dopamine was proposed based on the redox reaction between silver ion and dopamine, which was catalyzed by silver nanoparticles(AgNPs), causing the color change. The increase of dopamine concentration led to red shift of absorption peak and increase of the absorbance. Meanwhile, the color of the solution changed from pale yellow and deep yellow. Under optimal conditions, a linear range of 0.05—16 μmol/L between the absorbance and dopamine concentration and a detection limit of 0.04 μmol/L was obtained. The proposed method was sensitive, simple, low-cost and selective, which could be applied for the detection of dopamine in human serum.

Key words: Silver nanoparticles, Colorimetric, Dopamine

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