基于超疏水聚苯乙烯膜的蛋白质微液滴检测

doi:10.7503/cjcu20180106

高等学校化学学报 ›› 2018, Vol. 39 ›› Issue (9): 1913.doi: 10.7503/cjcu20180106

基于超疏水聚苯乙烯膜的蛋白质微液滴检测

徐丹¹, 丁亚丹¹, 王雪², 丛铁³, 刘俊平¹, 洪霞¹(), 潘颖²()

1. 紫外光发射材料与技术教育部重点实验室(东北师范大学), 长春 130024
2. 吉林大学中日联谊医院, 长春 130033
3. 大连理工大学盘锦校区石油与化学工程学院, 盘锦 124221

收稿日期:2018-02-04 出版日期:2018-05-15 发布日期:2018-05-15
作者简介:
联系人简介: 洪霞, 女, 博士, 副教授, 主要从事纳米生物医用材料研究. E-mail: xiahong@nenu.edu.cn; 潘颖, 女, 博士, 教授, 主要从事妇科肿瘤诊断及治疗研究. E-mail: panying424@163.com
基金资助:
国家自然科学基金(批准号: 51272040, 11604043)和吉林省教育厅十三五科技研究项目(批准号: JJKH20170910KJ)资助.

Microdroplet Detection of Protein Based on Superhydrophobic Polystyrene Film^†

XU Dan¹, DING Yadan¹, WANG Xue², CONG Tie³, LIU Junping¹, HONG Xia^1,^*(), PAN Ying^2,^*()

1. Key Laboratory of UV-Emitting Materials and Technology, Ministry of Education(Northeast Normal University), Changchun 130024, China
2. China-Japan Union Hospital of Jilin University, Changchun 130033, China
3. School of Petroleum and Chemical Engineering, Panjin Campus, Dalian University of Technology, Panjin 124221, China

Received:2018-02-04 Online:2018-05-15 Published:2018-05-15
Contact: HONG Xia,PAN Ying E-mail:xiahong@nenu.edu.cn;panying424@163.com
Supported by:
† Supported by the National Natural Science Foundation of China(Nos. 51272040, 11604043) and the Thirteenth Five-Year Science and Technology Research Project of Education Department of Jilin Province, China(No. JJKH20170910KJ).

摘要/Abstract

摘要：

利用静电纺丝法制备了具有低滚动角的超疏水聚苯乙烯膜, 建立了基于该超疏水膜的蛋白质微液滴检测方法. 超疏水表面确保了球状液滴的形成, 使待测样品量由传统方法的上百微升降至10 μL. 将双缩脲比色检测法与基于超疏水表面的微液滴检测技术相结合, 实现了微升量级的牛血清白蛋白(BSA)分子的快速定性及高灵敏定量检测. 由于微液滴在超疏水表面浓缩效果更佳, 因此检测下限由溶液相检测下限的24.53 μmol/L降低到1.490 μmol/L. 这种蛋白质微液滴检测技术具有一定的普适性. 为实现微量生物/化学分子的高灵敏检测提供了新的技术平台.

关键词: 静电纺丝, 超疏水聚苯乙烯膜, 低滚动角, 蛋白质微量检测

Abstract:

Superhydrophobic polystyrene film with low water sliding angle was prepared by electrospinning, and the microdroplet detection technology of protein based on the superhydrophobic film was established. The superhydrophobic surface ensures the formation of spherical droplets, and the amount of sample required was thus decreased from hundreds of microliters to 10 μL. The low water sliding angle facilitates the manipulation of microdroplets and recycling of the detection substrates. Rapidly qualitative and sensitively quantitative detection of bovine serum albumin(BSA) molecules was achieved by the combination of biuret colorimetric assay and the microdroplet detection technology based on the superhydrophobic surface. Compared with the solution phase detection, the detection limit was decreased from 24.53 μmol/L to 1.490 μmol/L after drying the microdroplet on the electrospining polystyrene(PS) film due to the better concentration effect of the analytes on the superhydrophobic surface. This microdroplet detection technology of protein has a certain degree of universality. This study provides a new technical platform for the highly sensitive detection of trace biological/chemical molecules.

Key words: Electrospinning, Superhydrophobic polystyrene film, Low water sliding angle, Microdroplet detection of protein

中图分类号:

O656
O631

TrendMD:

徐丹, 丁亚丹, 王雪, 丛铁, 刘俊平, 洪霞, 潘颖. 基于超疏水聚苯乙烯膜的蛋白质微液滴检测. 高等学校化学学报, 2018, 39(9): 1913.

XU Dan,DING Yadan,WANG Xue,CONG Tie,LIU Junping,HONG Xia,PAN Ying. Microdroplet Detection of Protein Based on Superhydrophobic Polystyrene Film^†. Chem. J. Chinese Universities, 2018, 39(9): 1913.

图/表 7

Fig.1 SEM images of the electrospining PS film(A) and smooth PS film(B) Inset is the magnification of (A).

Fig.2 Water contact angle of the smooth PS film(A) and the electrospining PS film(B) Inset in (B) is water sliding angle of the electrospining PS film.

Fig.3 Reaction product structure of protein and biuret reagent(A), the microdroplet detection photographs of BSA before(B) and after(C) the droplet of biuret solution and the droplet of BSA solution coalesced with different concentrations of BSA(from left to right: 1.490, 14.90, 149.00 and 1490.00 μmol/L)(D) and UV-Vis spectra of BSA(a), biuret(b) and their reaction product(c)(E)

Fig.4 Absorption spectra of biuret upon the addition of various concentrations of BSA(A), the relationship between the integral area of absorption and the concentration of the BSA(B), the absorption spectra of Biuret upon the addition of various concentrations of BSA after drying on the electrospining PS film(C) and the relationship between the integral area of absorption and the concentration of the BSA after drying on the electrospining PS film(D)The insets are a linear relationship of absorption integral area versus the concentration of BSA over the range of 29.80—119.2 μmol/L(B) and a linear relationship of absorption integral area versus the concentration of BSA after drying on the electrospining PS film over the range from 1.490 μmol/ to 149.0 μmol/L(D), respectively.

Fig.5 Photographs of the dried reaction product on hydrophobic surface(A) and superhydrophobic surface(B)

Fig.6 Absorption spectra of biuret upon the addition of various concentrations of BSA after drying on smooth PS film(A) and the relationship between the integral area of absorption and the concentration of the BSA after drying on smooth PS film(B)The inset in (B) is a linear relationship of absorption integral area versus the concentration of BSA after drying on smooth PS film over the range from 74.50 μmol/L to 149.0 μmol/L.

Fig.7 Photographs of the microdroplet detection in biuret solution and LZM solution(A), biuret solution and milk powder solution(C) and their coalesced droplet on superhydrophobic surface(B, D)

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基于超疏水聚苯乙烯膜的蛋白质微液滴检测

Microdroplet Detection of Protein Based on Superhydrophobic Polystyrene Film^†

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基于超疏水聚苯乙烯膜的蛋白质微液滴检测

Microdroplet Detection of Protein Based on Superhydrophobic Polystyrene Film†

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Microdroplet Detection of Protein Based on Superhydrophobic Polystyrene Film^†