高等学校化学学报 ›› 2018, Vol. 39 ›› Issue (1): 178-184.doi: 10.7503/cjcu20170336

• 高分子化学 • 上一篇    下一篇

纳米纤维增强双层组织引导膜的力学性能及细胞响应

王志微1,2, 黄棣1,2(), 席少晖1, 徐梦洁1, 朱雪芳1, 连小洁1, 魏延1,2(), 陈维毅2   

  1. 1. 太原理工大学力学学院生物医学工程系, 纳米生物材料与再生医学研究中心 太原 030024
    2. 应用力学与生物医学工程研究所, 材料强度与结构冲击山西省重点实验室, 太原 030024
  • 收稿日期:2017-05-31 出版日期:2018-01-10 发布日期:2017-09-18
  • 作者简介:联系人简介: 黄 棣, 男, 博士, 副教授, 主要从事纳米生物材料研究. E-mail:huangjw2067@163.com;魏 延, 男, 博士, 讲师, 主要从事纳米生物材料研究. E-mail:weiyanbme@gmail.com
  • 基金资助:
    国家自然科学基金(批准号: 11502158, 51503140, 11632013, 51502192)和山西省青年科技基金(批准号: 2015021195)资助

Cell Response and Mechanical Properties of the Nanofibers Reinforced Bilayered Tissue Guided Memberanes

WANG Zhiwei1,2, HUANG Di1,2,*(), XI Shaohui1, XU Mengjie1, ZHU Xuefang1, LIAN Xiaojie1, WEI Yan1,2,*(), CHEN Weiyi2   

  1. 1. Research Center for Nano-biomaterials & Regenerative Medicine, Department of Biomedical Engineering, College of Mechanics, Taiyuan 030024, China
    2. Institute of Applied Mechanics & Biomedical Engineering, Shanxi Key Labratory of Materials Strength & Structrual Impact, Taiyuan University of Technology, Taiyuan 030024, China
  • Received:2017-05-31 Online:2018-01-10 Published:2017-09-18
  • Contact: HUANG Di,WEI Yan E-mail:huangjw2067@163.com;weiyanbme@gmail.com
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos.11502158, 51503140, 11632013, 51502192) and the Natural Science Foundation of Shanxi Province, China(No.2015021195)

摘要:

通过热致相分离结合静电纺丝技术, 在纳米羟基磷灰石/聚酰胺(nHA/PA)多孔膜基底表面形成不同取向的PA纳米纤维, 制备了纤维增强双层组织引导膜. 研究了不同转速(0, 500, 1500 r/min) 下, nHA/PA多孔膜表面形成的PA纳米纤维的微观结构及取向情况对力学性能的影响. 将双层组织引导膜与MG63细胞共培养, 研究细胞在双层组织引导膜周围的生长情况, 并通过四甲基偶氮唑盐比色法(MTT)定量研究了细胞的增殖情况. 结果表明, 热致相分离法制备的nHA/PA多孔膜表面及内部富含多孔结构, nHA晶粒在PA基质中均匀分布. 采用静电纺丝法在nHA/PA多孔膜表面构建纳米纤维, 随着转速增大, nHA/PA多孔膜表面的PA纳米纤维取向性逐渐增加. 引入PA纳米纤维后, 双层组织引导膜的力学性能均有增强, 在500 r/min转速下, 双层组织引导膜中, 纳米纤维层与nHA/PA基底应变同时达到最大, 拉伸强度达到(39.86±4.73) MPa. 细胞实验结果显示, 双层组织引导膜周围的细胞生长良好, 增殖明显, 具有良好的细胞相容性, 在牙周组织缺损再生领域有潜在应用前景.

关键词: 静电纺丝, 双层组织引导膜, 力学性能, 细胞相容性, 聚酰胺纳米纤维

Abstract:

The bilayer guiding bone regeneration(GBR) membrane used for simultaneous periodontal regeneration was fabricated. Firstly, nano hydroxyapatite/polyamide(nHA/PA) membrane was prepared by thermally induced phase separation. Afterwards, PA nanofibers with different orientation was formed by electrospinning on the surface of the porous nano hydroxyapatite/polyamide(nHA/PA) membrane. The microstructure, orientation and mechanical properties of the PA nanofibers were investigated under different rotational speed(0, 500, 1500 r/min). The results show that porous structure is presented on the surface and inside of the nHA/PA membrane. The nHA crystals distribute uniformly in the PA matrix. The orientation of the PA nanofibers is improved with the increasing of rotation speed. The mechanical testing results show that the elastic strength of the bilayer construct can reach(39.86±4.73) MPa under the rotation speed of 500 r/min. The cell culture results show that the MG63 cells grow well around the bilayer construct. The bilayer constructs display favorable cytocompatibility and show potential clinical applications in the field of periodontal regeneration.

Key words: Electrospining, Bilayered tissue guided memberane, Mechanical property, Cytocompatibility, Polyamide nanofiber

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