高等学校化学学报 ›› 2018, Vol. 39 ›› Issue (3): 575-582.doi: 10.7503/cjcu20170491

• 高分子化学 • 上一篇    下一篇

乳液静电纺丝PLA/PEG微纳纤维膜的可控制备及负载亲疏水性药物的释放

高燕, 张华(), 张雯(), 李祥鹏, 岳盼, 李伟   

  1. 天津市先进纤维与储能技术重点实验室, 天津工业大学材料科学与工程学院, 天津300387
  • 收稿日期:2017-07-19 出版日期:2018-03-10 发布日期:2018-01-13
  • 作者简介:联系人简介: 张 华, 女, 博士, 教授, 主要从事纳米材料研究. E-mail:hua1210@126.com; 张 雯, 女, 博士, 讲师, 主要从事药物缓释载体材料研究. E-mail:zhangwen2050@hotmail.com
  • 基金资助:
    国家自然科学基金(批准号: 51573135)、 天津市高等学校科技发展基金(批准号: 20140305) 和天津市应用基础与前沿技术研究计划项目(批准号: 16JCYBJC17100)资助

Preparation of Micro/Nano-fibers Membranes Encapsulated with Dual Drugs by Emulsion Electrospun and Controlled Release of Hydrophilic and Hydrophobic Drugs

GAO Yan, ZHANG Hua*(), ZHANG Wen*(), LI Xiangpeng, YUE Pan, LI Wei   

  1. Tianjin Key Laboratory of Advanced Fibers and Energy Storage, Institute of Functional Fibers,Materials Science and Engineering of Tianjin Polytechnic University, Tianjin 300387, China
  • Received:2017-07-19 Online:2018-03-10 Published:2018-01-13
  • Contact: ZHANG Hua,ZHANG Wen E-mail:hua1210@126.com;zhangwen2050@hotmail.com
  • Supported by:
    † Supported by the National Nature Science Foundation of China(No.51573135), the Tianjin Colleges and Universities of Science and Technology Development Fund, China(No.20140305) and the Tianjin Research Program of Application Foundation and Advanced Technology, China(No.16JCYBJC17100)

摘要:

采用低能相反转法, 以聚乳酸(PLA)、 疏水性药物喜树碱(CPT)溶液为油(O)相, 以明胶水溶液、 亲水性药物黄芪多糖(APS)为水(W)相, 制备水包油(O/W)初乳液. 通过控制聚乙二醇(PEG)的浓度和分子量制备O/W纺丝液, 经乳液静电纺丝获得PLA/PEG微纳纤维膜. 采用粒径分布、 光学显微镜(OM)、 扫描电子显微镜(SEM)、 红外光谱(FTIR)、 X射线衍射(XRD)、 接触角测试和细胞毒性实验对初乳液和PLA/PEG微纳纤维膜进行表征, 并通过激光共聚焦显微镜(CLSM)观察药物的分布情况. 结果表明, 通过乳液静电纺丝可成功制备亲水性良好的不同微纳结构的PLA/PEG微纳纤维膜. PLA/PEG微纳纤维膜形貌不同, 亲水性存在差异, 无细胞毒性. 体外药物释放结果表明, 与pH=6.8和7.4的释放介质相比, 在pH=5.8的释放介质中, 药物累积释放率较高, 表明载药PLA/PEG微纳纤维膜能够有效减缓CPT的释放, 而APS释放速率较快, 可实现亲疏水性药物的差别性释放.

关键词: 乳液静电纺丝, 相反转, 聚乳酸/聚乙二醇微纳纤维, 差别性释放

Abstract:

Polylactic acid(PLA) and hydrophobic drug of camptothecin(CPT) solution play as the oil(O) phase. At the same time, gelation solution with hydrophilic drug of astragalus polysccharide(APS) play as the water(W) phase. Oil-in-water(O/W) emulsion was prepped by phase reversion method which was the method of low energy. The viscosity of O/W emulsion was adjusted by controlling the concentration and molecular weight of polyethlene glycol(PEG). The PLA/PEG micro/nano-fibers membrane was obtained by emulsion electrospun. On the one hand, the emulsion was characterized by particle size, optical microscope(OM). On the other hand, the scanning electromicroscope(SEM), infrared spectroscopy(FTIR) and X-ray diffraction(XRD) were used to characterize the structure and ingredient of the PLA/PEG micro/nano-fibers membrane. Besides, the hydrophilia and cytotoxicity test were used to evaluate the biocompatibility of PLA/PEG micro/nano-fibers membrane. In order to observe the distribution of drugs, the laser scanning confocal microscopy(CLSM) was used. The results show that different micro-nano structure of PLA/PEG micro/nano-fibers can be successful spinning by emulsion electrospun. Even though different morphology of composite membrane had different hydrophilia, but its hydrophilia was good. At the same time, PLA/PEG micro/nano-fibers membrane had a good biocompatibility with no cytotoxicity. The in vitro release results showed that the accumulative release of drugs in the phosphate buffer solution of pH=5.8 was higher than in the phosphate buffer solution of pH=6.8 and pH=7.4 so that the phosphate buffer solution of pH=5.8 was more advantageous to the release of drugs. As well as we can conclude that APS release faster than CPT. The PLA/PEG micro/nano-fibers membrane can realize differential release of drugs in phosphate buffer solution of pH=5.8.

Key words: Emulsion electrospun, Phase reversion, Polylactic acid/polyethylene glycol micro/nano-fiber, Differential release

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