高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (4): 851.

• 研究论文 • 上一篇    下一篇

药物与细胞膜相互作用的ppKCE动力学参数

夏之宁1,2,李丽仙1,2,陈华2,熊彩侨3,杨丰庆2   

  1. 1. 重庆大学生物工程学院;
    2. 化学化工学院, 重庆 400030;
    3. 中国科学院化学研究所, 北京 100190
  • 收稿日期:2010-07-13 修回日期:2010-08-23 出版日期:2011-04-10 发布日期:2011-03-09
  • 通讯作者: 夏之宁 E-mail:chem_lab_cqu@yahoo.com.cn
  • 基金资助:

    国家自然科学基金(批准号: 20775096, 20805060)资助.

Kinetic Parameters of Interaction Between Cell Membrane and Drug by ppKCE

XIA Zhi-Ning1,2*, LI Li-Xian1,2, CHEN Hua2, XIONG Cai-Qiao3, YANG Feng-Qing2   

  1. 1. College of Bioengineering,
    2. College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, China;
    3. Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
  • Received:2010-07-13 Revised:2010-08-23 Online:2011-04-10 Published:2011-03-09
  • Contact: XIA Zhi-Ning E-mail:chem_lab_cqu@yahoo.com.cn
  • Supported by:

    国家自然科学基金(批准号: 20775096, 20805060)资助.

摘要: 本文在区段-区段动力学毛细管电泳(ppKCE)的理论基础上,以兔红细胞膜、人红细胞膜为受体,首次同时测得了表征药物与细胞膜间结合快慢的正向结合速率常数kon、反向解离速率常数koff、以及配体与受体间相互作用的结合常数Kb。实验中考察了迁移时间及峰高的稳定性,pH值对药物与细胞膜相互作用动力学参数的影响。该方法为评价药物疗效、毒性及药物动力学研究提供一种新的方法。

关键词: 区段-区段动力学毛细管电泳(ppKCE), 细胞膜, 动力学参数, 相互作用

Abstract: Based on the theory of plug-plug kinetic capillary electrophoresis (ppKCE), the rabbit red blood cell membrane and human red blood cell membrane were used to study the kinetic parameters of the interaction between the cell membrane and drug for the first time. The rate constants of complex formation kon, the dissociation koff and the binding constant Kb between cell membrane and drugs were directly determined simultaneously. The stability of the peaks height and the migration times were studied, and the influence of pH on the kinetic parameters of the interaction between the cell membrane and drugs were examined and analyzed. It could provide a new approach for the evaluation of drug efficiency, toxicity and pharmacokinetics in vivo.

Key words: Plug-plug kinetic capillary electrophoresis(ppKCE), Cell membrane, Kinetic parameter, Interaction

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