高等学校化学学报

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (12): 2828.

• 研究论文 • 上一篇    下一篇

壳聚糖季铵盐-Zn(Ⅱ)催化磷酸双酯与质粒DNA水解动力学

彭英桂1,张琦2,相艳1,2   

  1. 1. 北京航空航天大学化学与环境学院,
    2. 材料科学与工程学院, 北京 100191
  • 收稿日期:2011-03-22 修回日期:2011-05-04 出版日期:2011-12-10 发布日期:2011-11-25
  • 通讯作者: 相艳 E-mail:xiangy@buaa.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 21073010)资助.

Kinetics of Catalytic Hydrolysis of Phosphodiester and Plasmid DNA by the Quaternary Ammonium Salt of Chitosan with Zn(Ⅱ)

PENG Ying-Gui1, ZHANG Qi2, XIANG Yan1,2*   

  1. 1. School of Chemistry and Environment,
    2. School of Materials Science and Engineering, Beihang University, Beijing 100191, China
  • Received:2011-03-22 Revised:2011-05-04 Online:2011-12-10 Published:2011-11-25
  • Contact: XIANG Yan E-mail:xiangy@buaa.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 21073010)资助.

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摘要： 采用壳聚糖与缩水甘油三甲基氯化铵反应制备了壳聚糖季铵盐(HTACC), 研究了其Zn(Ⅱ)配合物HTACC-Zn(Ⅱ)催化DNA的模拟底物对硝基苯酚磷酸双酯(BNPP)水解的动力学过程及其对质粒DNA的催化裂解. 结果表明, HTACC-Zn(Ⅱ)对BNPP的水解反应具有良好的催化活性, 其表观一级速率常数可达到6.7×10-6 s-1, 为BNPP自发水解时的6.0×104倍; 同时, HTACC-Zn(Ⅱ)还能够有效催化质粒DNA(pUC19)的裂解, 使DNA分子由超螺旋结构裂解为开环和线型结构.

关键词: 壳聚糖, 季铵盐, 磷酸双酯, 催化水解, DNA裂解

Abstract: The hydroxypropyl trimethyl ammonium chloride chitosan(HTACC) was synthesized by the reaction of chitosan with glycidyl trimethylammonium chloride. The kinetics of hydrolysis of bis(p-nitrophenyl)phosphate(BNPP) catalyzed by HTACC-Zn(Ⅱ) in Tris-H+ buffer was studied and analyzed. In addition, the cleavage of plasmid DNA and the molecular interaction with HTACC-Zn(Ⅱ) were also investigated. The results show that the catalyst has fine catalytic activity to the hydrolysis of phosphodiesters with a first-order rate constant of kobs＝6.7×10-6 s-1, which is 6.0×10-4 folds of its spontaneous hydrolysis. Furthermore, the catalyst can cleavage plasmid DNA(pUC19) from supercoiled form to nicked form and/or linear form effectively.

Key words: Chitosan, Quaternary ammonium salt, Phosphodiester, Catalytic hydrolysis, DNA cleavage

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