高等学校化学学报

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (12): 2354.

• 研究论文 • 上一篇    下一篇

羧基化氧化石墨烯的血液相容性

徐东1,2,周宁琳1,2,3,沈健1,2,3   

  1. 1. 南京师范大学化学与材料科学学院, 江苏省生物医药功能材料工程研究中心,  南京 210097;
    2.  南京师范大学江苏省生物功能材料重点实验室, 南京 210097;
    3. 南京大学江苏省表面与界面工程技术研究中心, 南京 210093
  • 收稿日期:2010-03-01 修回日期:2010-06-11 出版日期:2010-12-10 发布日期:2010-12-06
  • 通讯作者: 周宁琳 E-mail:zhouninglin@njnu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:  20874047), 江苏省自然科学基金(批准号:  BK2009408)和“211工程”重点学科建设项目基金资助.

Hemocompatibility of Carboxylic Graphene Oxide

XU Dong1,2,  ZHOU NingLin1,2,3*,  SHEN Jian1,2,3   

  1. 1.  Jiangsu Engineering Research Center for Biomedical Function Materials,  College of Chemistry and Materials Science, Nanjing Normal University,  Nanjing 210097,  China
    2.  Jiangsu Key Laboratory of Biofunctional Materials,   Nanjing Normal University,  Nanjing 210097,  China;
    3.  Jiangsu Technological Research Center for Interfacial Chemistry and Chemical Engineering,  Nanjing University,  Nanjing 210093,  China  
  • Received:2010-03-01 Revised:2010-06-11 Online:2010-12-10 Published:2010-12-06
  • Contact: ZHOU Ning-Lin E-mail:zhouninglin@njnu.edu.cn
  • Supported by:

    Province Natural Science Foundation of Jiangsu;National Natural Science Foundation of China

PDF (PC)

被引次数

56

摘要： 氧化石墨烯的官能团修饰可促进氧化石墨烯与生物体液、聚合物基体等不同环境的融合. 通过超声法制备了氧化石墨烯, 并利用化学改性的方法, 将氧化石墨烯表面的羟基和环氧基转变为羧基. 红外图谱上羧基化氧化石墨烯(GeneO-COOH)的羧基振动明显, 峰强增大. GeneO-COOH的主要失重表现为羧基官能团缩合以一个水分子的形态释放失去结构水[OH2]. 复钙动力学曲线随着GeneO-COOH的浓度增加, 曲线上升趋势由陡峭趋于平缓, 当浓度为1.25 μg/mL时复钙时间延长了11 min, 平台期OD值降低了8.14 %; 在0.5~100 μg/mL浓度范围内溶血率均<5 %. GeneO-COOH比GeneO在同等低浓度下的抗凝血性能有一定程度的改善, 主要是因为带负电的羧基可直接络合凝血因子. 因此, 羧基修饰氧化石墨烯是提高血液相容性的有效手段, 羧基化氧化石墨烯可作为潜在的生物医用材料的填料.

关键词: 羧基, 表面改性, 氧化石墨烯, 血液相容性

Abstract: Functionalization of Graphene oxide is important for technological applications since it allows integrating into different environments such as biological fluids, polymer matrices, silicon surface and others. Graphene oxide was made through the ultrasonic separation of graphite oxide, and then it was modified with functional group by chloroacetic acid, and finally, the graphene oxide modified with carboxyl groups was prepared. Compared with graphene oxide, the FTIR of GeneO-COOH showed a weaker -OH peak, a stronger -COOH peak, and a bigger peak width. FTIR showed partial epoxy and hydroxyl were activated into carboxyl. On TG spectrum, the main weight loss of GeneO-COOH showed condensation of -COOH groups in the form of a loss of structural water. The curve which reflected the kinetic profiles of recalcification time was changed from steep to gentle along with the increases of the consistency of GeneO-COOH. The recalcification time of GeneO-COOH was prolonged by 11 min at 1.25μg/mL, and the value of OD reduced by 8.14%. The hematolysis ratio was less than 5 % within the range of 0.5~100μg/mL. The anti-clotting property of GeneO-COOH was better than that of GeneO at the same low concentration. The improvement was mainly made by carboxyl group with a negative charge and direct complexation between carboxyl group and clotting factor. Hence, it may be conclude that GeneO-COOH may be suitable as biomaterials in the field of medical.

Key words: arboxyl group, surface modification, graphene oxide, hemocompatibility

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