高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

微过氧化物酶-11在壳聚糖修饰玻碳电极上的电化学

王琨琦1,3,4, 马中苏1, 陆天虹3, 邢巍3, 朱琳3, 吕强1, 邢沈阳2
  

    1. 吉林大学生物与农业工程学院, 长春 130025;
    2. 吉林大学畜牧兽医学院, 长春 130062;
    3. 中国科学院长春应用化学研究所, 长春 130022;
    4. 长春工程学院理学院, 长春 130021
  • 收稿日期:2008-06-04 修回日期:1900-01-01 出版日期:2009-03-10 发布日期:2009-03-10
  • 通讯作者: 马中苏

Electrochemistry of Microperoxidase-11(MP-11) on Glassy Carbon Electrode Modified with Chitosan

WANG Kun-Qi1,3,4, MA Zhong-Su1*, LU Tian-Hong3, XING Wei3, ZHU Lin3, LÜ Qiang1, XING Shen-Yang2   

    1. College of Biological & Agricultural Engineering, Jilin University, Changchun 130025, China;
    2. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China;
    3. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    4. School of Science, Changchun Institute of Technology, Changchun 130021, China
  • Received:2008-06-04 Revised:1900-01-01 Online:2009-03-10 Published:2009-03-10
  • Contact: MA Zhong-Su

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被引次数

7

摘要： 采用物理吸附法, 将微过氧化物酶-11(MP-11)固定在载体壳聚糖修饰的玻碳电极表面. 运用循环伏安法对MP-11在该修饰电极上的直接电化学行为及对氧(O2)和过氧化氢(H2O2)的电催化行为进行了表征. 研究结果表明, 在pH=7.12的磷酸盐缓冲溶液中, 壳聚糖修饰电极上的MP-11发生了准可逆的氧化还原反应, 而且在反应过程中包含质子的传递过程, 完全实现了MP-11在该修饰电极上的直接电化学. 该修饰电极也可以对O2和H2O2进行电催化还原, 并且两个反应的电催化还原过程都是受表面控制的电化学过程, 对H2O2催化还原产生的响应电流与H2O2的浓度呈线性关系.

关键词: 微过氧化酶-11, 壳聚糖, 电催化

Abstract: In this paper, microperoxidase-11(MP-11) was immobilized on glassy carbon electrode surface modified with chitosan by physical adsorption. The direct electrochemistry and the electrocatalytic behaviours to O2 and the H2O2 of MP-11 on glassy carbon electrode modified with chitosan were characterized by cyclic voltammetry. The results indicate that MP-11 on modified electrode displays a quasi-reversible electrochemical process coupled with proton transfer in the phosphate buffer solutions(pH=7.12). Direct electrochemical reaction of MP-11 on modified electrode has been realized. MP-11 on modified electrode can catalyze reduction for O2 and H2O2. Both of the catalytic reductions are surface-controlled electrochemical process. The response current of MP-11 on modified electrode to H2O2 is linear with hydrogen peroxide concentration. In a word, the electrode modified by chitosan as a matrix material can be used in the biofuell cell and biosensor in the future.

Key words: Microperoxidase-11(MP-11), Chitosan, Electrocatalysis

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