高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

3-氨基苯硼酸为功能单体在壳聚糖上印迹牛血清白蛋白的研究

王华芳1, 何运华1, 何锡文1, 李文友1, 陈朗星1, 张玉奎1,2   

    1. 南开大学化学系, 天津 300071;
    2. 中国科学院大连化学物理研究所, 大连 116011
  • 收稿日期:2007-06-27 修回日期:1900-01-01 出版日期:2008-04-10 发布日期:2008-04-10
  • 通讯作者: 何锡文,张玉奎

Study of Bovine Serum Albumin Imprinted Polymer Fabricated with 3-Aminophenylboronic Acid as Functional Monomer on Chitosan Particles

WANG Hua-Fang1, HE Yun-Hua1, HE Xi-Wen1*, LI Wen-You1, CHEN Lang-Xing1, ZHANG Yu-Kui1,2*   

    1. Department of Chemistry, Nankai University, Tianjin 300071, China;
    2. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China
  • Received:2007-06-27 Revised:1900-01-01 Online:2008-04-10 Published:2008-04-10
  • Contact: HE Xi-Wen,ZHANG Yu-Kui

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被引次数

43

摘要： 以壳聚糖为载体, 3-氨基苯硼酸为功能单体对牛血清白蛋白进行了分子印迹的研究, 并对吸附过程进行Langmuir等温吸附模型的数据处理. 结果表明, 印迹聚合物上形成了对于模板分子有较高的吸附容量和选择性的识别位点, Langmuir等温吸附平衡常数为49.5 mL/mg, 结合位点的最大表观结合量为16.3 mg/g, 证明了该印迹聚合物对于牛血红蛋白和溶菌酶这些非模板蛋白的吸附不具有选择性.

关键词: 3-氨基苯硼酸, 牛血清白蛋白, 壳聚糖, 分子印迹技术

Abstract: 3-Aminophenylboronic acid was used as functional monomer, molecularly imprinted polymer of bovine serum albumin was fabricated on chitosan particles. The Langmuir adsorption model was undertaken to describe the equilibrium isotherms. Analysis of the model shows that many recognition sites of BSA were formed on the MIP with higher adsorption capacity and higher selectivity respect to the other two candidates. Langmuir adsorption equilibrium constant was 49.5 mL/mg and the theoretical maximum adsorption capacity was 16.3 mg/g. The imprinted polymers showed a better selectivity and capacity of the template than one of other competitive proteins. Therefore, this strategy and the functional monomer are promising in molecularly imprinting technology for separating and purifying each protein component in complex bio-samples.

Key words: 3-Aminophenylboronic acid, Bovine serum albumin, Chitosan, Molecularly imprinted technology

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