高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

柘树根多糖的分离纯化及结构表征

石磊1,2, 陈靠山1, 董群2, 方积年2, 丁侃2   

    1. 山东大学生命科学学院, 济南 250100;
    2. 中国科学院上海药物研究所, 上海 201203
  • 收稿日期:2006-07-12 修回日期:1900-01-01 出版日期:2007-06-10 发布日期:2007-06-10
  • 通讯作者: 丁侃

Separation, Purification and Structure Characterization of Polysaccharide from Roots of Cudrania tricuspidata(Carr.) Bur.

SHI Lei1,2, CHEN Kao-Shan1, DONG Qun2, FANG Ji-Nian2, DING Kan2*   

    1. School of Life Science, Shandong University, Jinan 250100, China;
    2. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
  • Received:2006-07-12 Revised:1900-01-01 Online:2007-06-10 Published:2007-06-10
  • Contact: DING Kan

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被引次数

31

摘要： 以柘树[Cudrania tricuspidata(Carr.) Bur.]的根为材料, 经热水抽提、木瓜蛋白酶-Sevag法除蛋白、乙醇沉淀和DEAE-Sephadex A-50凝胶柱层析分离纯化, 得到一种水溶性的柘树根多糖(CPS-0). 采用HPLC、糖基组成分析、甲基化分析、GC、GC-MS、NMR(1H NMR, 13C NMR及HMQC)、元素分析、UV和IR等技术对CPS-0的纯度、性质、组成和结构进行表征. 结果表明, CPS-0仅含葡萄糖, 分子量为4.6×103, 主链由1,4-连接的α-D-葡萄糖残基组成, 其侧链由末端及1,4-连接的葡萄糖残基构成, 取代于主链分支点葡萄糖的6位, 平均每10个葡萄糖残基组成的重复单元中含有1个分支.

关键词: 柘树根, 多糖, 分离纯化, 结构表征

Abstract: A water-soluble polysaccharide(CPS-0) was obtained from the root of Cudrania tricuspidata(Carr.) Bur., by hot water extraction(70 ℃), deproteination with enzymolysis and Sevag method, precipitation with ethanol, and fractionation through DEAE-Sephadex A-50 chromatography. The purity of CPS-0 was determined via HPLC and the structure was characterized with monosaccharide composition analysis, methylation analysis, GC, GC-MS, NMR spectra(1H NMR, 13C NMR, HMQC), UV, IR, and elemental analysis. The CPS-0 was found to contain glucose residue solely, with an average repeating unit of decasaccharide, ha-ving a backbone consisting of 1,4-linked α-D-glucopyranosyl residues, to which the side chain consisting of terminal and 1,4-linked α-D-glucopyranosyl residues was attached at position 6 of the branching residues.

Key words: Root of Cudrania tricuspidata(Carr.) Bur., Polysaccharide, Separation and purification, Structure characterization

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