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微乳液电动色谱测定油-水分配系数的改进方法研究

蒋雪梅1, 2, 魏为力1, 姜军坡1, 石开云2, 穆小静1, 陈志涛2, 夏之宁1, 2   

    1. 重庆大学化学化工学院药学系,
    2. 生物力学与组织工程教育部重点实验室, 重庆 400044
  • 收稿日期:2005-12-15 修回日期:1900-01-01 出版日期:2006-08-10 发布日期:2006-08-10
  • 通讯作者: 夏之宁

Measurement of Oil-Water Partition Coefficients by Improved Microemulsion Electrokinetic Chromatography

JIANG Xue-Mei1,2, WEI Wei-Li1, JIANG Jun-Po1, SHI Kai-Yun2, MU Xiao-Jing1, CHEN Zhi-Tao2, XIA Zhi-Ning1,2   

    1. Department of Pharmaceutics, Institute of Chemistry and Chemical Engineering,
    2. Key Laboratory for Biomechanics and Tissue Engineering, Ministry of Education, Chongqing University, Chongqinq 400044, China
  • Received:2005-12-15 Revised:1900-01-01 Online:2006-08-10 Published:2006-08-10
  • Contact: XIA Zhi-Ning

摘要: 通过考察微乳液电动色谱(MEEKC)中化合物浓度和高电场对其迁移行为的影响, 探讨了化合物分配于微乳后改变微乳内相性质的原因, 以及电泳高电场致使微乳液性质变化的机理. 在此基础上建立了一种测定化合物油-水分配系数的改进型MEEKC方法. 将此改进方法应用于烷基苯化合物之油-水分配系数的测定, 其测定值与文献参考值平均相差0.07个对数单位, 准确度较现行MEEKC方法有了明显提高.

关键词: MEEKC, 改进方法, 油-水分配系数, 高电场

Abstract: Microemulsion electrokinetic chromatography(MEEKC) was used as a powerful method for the indirect measurement of oil-water partition coefficients of organic compounds. Considering the problems of determining oil-water partition coefficients by conventional MEEKC, the concentration of solute and high electric-field having impact on the migration behavior of compound was studied, the reason and mechanism of which were also studied. Moreover, an improved method of MEEKC(I-MEEKC) that can be used to solve the problems found was developed. Applying this improved method to determining oil-water partition coefficients for compounds, such as alkyl-benzene: benzene, toluene and ethyl benzene, good result was obtained: the average error between oil-water partition coefficients estimated by using improved MEEKC and literature values published was 0.07 logarithm units. In this work not only a new method was established to measure oil-water partition coefficients with MEEKC, but also the improved MEEKC is developed further and become more reliable and more accurate method for qualitative analysis.

Key words: MEEKC, Improved method, Oil-water partition coefficients, High electric-field

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