高等学校化学学报

高等学校化学学报 ›› 2006, Vol. 27 ›› Issue (3): 567.

• 研究论文 • 上一篇    下一篇

环状磷酸酯的酶促开环聚合

冯俊,王鹏,李峰,贺枫,卓仁禧   

  1. 武汉大学国家教育部生物医用高分子重点实验室,武汉大学化学与分子科学学院,武汉 430072
  • 收稿日期:2005-03-14 出版日期:2006-03-10 发布日期:2006-03-10
  • 通讯作者: 卓仁禧(1931年出生),男， 教授， 博士生导师, 中国科学院院士, 主要从事生物医用材料研究. E-mail: bmp_lab@hotmail.com
  • 基金资助:

    国家自然科学基金(批准号: 20304010)资助

FENG Jun, WANG Peng, LI Feng, HE Feng, ZHUO Ren-Xi   

  1. Department of Chemistry,  Wuhan University,  Wuhan 430072, China
  • Received:2005-03-14 Online:2006-03-10 Published:2006-03-10

PDF (PC)

被引次数

8

摘要：

研究了环状磷酸酯的酶促开环聚合反应,讨论了环状磷酸酯取代烷基对于酶促开环聚合及相应聚磷酸酯性能的影响,发现烷基取代基长度对于聚合度没有明显影响; 但随着环状磷酸酯的取代基长度增加,产率随之降低,聚磷酸酯的亲脂性增强. 猪胰脂肪酶和假丝酵母皱褶酶显示出比碱性磷脂酶更高的活性.

关键词: 聚磷酸酯; 酶促聚合; 开环聚合

Abstract:

Enzymatic ring-opening polymerization of cyclic phosphate was achieved in this study.  NMR spectra experiments showed that the  polyphosphate prepared by CL-catalyzed ring-opening polymerization had hydroxy and phosphoric acid end-groups at both termini.  The influence of different reaction factors such as reaction temperature, enzyme concentration and polymerization time was studied.  Six phosphates with different alkyl groups were polymerized in bulk by using three different enzymes.  The result indicated that the length of alkyl groups made no marked influence on  Dp but affected the yield greatly.  The longer the length of alkyl groups, the stronger the lipophilicity of the polyphosphates is.  Porcine pancreas lipase(PPL) and candida rugosa lipase(CL) exhibited better activity than akaline phosphatase(AP) in this polymerization reaction.

Key words: Polyphosphate; Enzymatic polymerization; Ring-opening polymerization

中图分类号: 

TrendMD: