高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (7): 1321.

• 研究论文 • 上一篇    下一篇

核磁共振技术及模式识别对硝酸镥急性毒性的研究

吴惠丰, 张晓宇, 李晓晶, 孙国英, 吴亦洁, 裴奉奎   

  1. 中国科学院长春应用化学研究所, 长春130022
  • 收稿日期:2004-06-23 出版日期:2005-07-10 发布日期:2005-07-10
  • 通讯作者: 裴奉奎(1940年出生),男,研究员,博士生导师,从事现代核磁共振波谱技术研究.E-mail:peifk@ciac.jl.Cn E-mail:peifk@ciac.jl.Cn
  • 基金资助:

    国家自然科学基金(批准号:29890280)资助.

Studies on the Acute Toxicity of Lu(NO3)3 by 1H NMR and Pattern Recognition Method

WU Hui-Feng, ZHANG Xiao-Yu, LI Xiao-Jing, SUN Guo-Ying, WU Yi-Jie, PEI Feng-Kui   

  1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  • Received:2004-06-23 Online:2005-07-10 Published:2005-07-10

摘要: 通过分析Wistar大鼠灌胃0.2,2,10和100mg/kg体重硝酸镥[Lu(NO3)3]48h内尿液的1HNMR谱,研究了重稀土化合物Lu(NO3)3在大鼠体内的急性生物效应.以氯化汞、铬酸钠、四氯化碳、盐酸肼和异硫氰酸-α-萘酯为模型药物,利用模式识别技术解析大鼠尿液的1HNMR谱,对不同剂量Lu(NO3)3的生物效应进行了分类.结果表明,应用核磁共振和模式识别相结合的方法,可清楚地认识稀土化合物生物效应,低剂量Lu(NO3)3(0.2,2mg/kg体重)的毒性与肾毒化合物铬酸钠类似,而高剂量Lu(NO3)3(10,100mg/kg体重)归入肝毒化合物四氯化碳组.该方法也可用于其它金属化合物及中药等药物的毒理学研究.

关键词: 稀土, 体液, 核磁共振, 模式识别, 毒性

Abstract: 1HNMR spectroscopy and pattern recognition method were used to assess acute toxicological effects of rare earth. Male Wistar rats were treated with Lu(NO3)-3 at doses of 0.2, 2.0, 10 and 100 mg/kg body weight with NaCrO4, HgCl2, CCl4, ANIT and N2H45HCl as the model toxins. The urine samples from the rats dosed with these compounds were collected over a 48 h time course and analyzed by 600 MHz 1HNMR spectra. Each NMR spectrum was data-processed to provide 220 intensity-related descriptors of spectra. The urine spectral data were analyzed by SIMCA method and successful classification of the toxicity and biochemical effects of Lu(NO3)3 were achieved. The toxicity induced by lower doses(0.2 and 2 mg/kg body weight) of Lu(NO3)3 was classified to NaCrO4 which produced lesion to proximal convoluted tubule cells, and the higher doses(10 and 100 mg/kg body weight) of Lu(NO3)3 could cause liver centrilobular necrosis like CCl4.

Key words: Rare earth, Biofluid, NMR spectroscopy, Pattern recognition, Toxicity

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