高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (4): 677.

• 研究简报 • 上一篇    下一篇

尿毒症中分子毒物的分离鉴定

杨眉1, 张建达1, 王芃1, 刘晓航1, 袁直1, 刘宏富2, 杨洪涛3, 杨淑芬2, 赵菁丽3   

  1. 1. 吸附分离功能高分子材料国家重点实验室, 南开大学高分子化学研究所, 天津 300071;
    2. 天津市天和医院, 天津 300050;
    3. 天津市中医学院第一附属医院, 天津 300193
  • 收稿日期:2004-05-14 出版日期:2005-04-10 发布日期:2005-04-10
  • 通讯作者: 袁　直(1961年出生),女,博士,教授,博士生导师,从事生物医用材料研究.E-mail:zhiy@nankai.edu.cn E-mail:zhiy@nankai.edu.cn
  • 基金资助:

    国家“九七三”计划(批准号:G1999064707)资助.

Separation and Identification of the Uremic Middle Molecules

YANG Mei1, ZHANG Jian-Da1, WANG Peng1, LIU Xiao-Hang1, YUAN Zhi1, LIU Hong-Fu2, YANG Hong-Tao3, YANG Shu-Fen2, ZHAO Jing-Li3   

  1. 1. The State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China;
    2. Tianhe Hospital, Tianjin 300050, China;
    3. First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
  • Received:2004-05-14 Online:2005-04-10 Published:2005-04-10

PDF (PC)

被引次数

2

关键词: 尿毒症, 中分子, 高效液相色谱, 飞行时间质谱

Abstract: Sera and urine from patients with severe uremia and healthy persons were separated by means of gel permeation chromatography on Sephadex G-15 column with N(C2H5)3-H2CO3 buffer as the eluent. Two middle molecular peaks(A and B) were detected at 206 nm in normal urine, uremic serum and uremic urine, but these two peaks were hardly observed in the profile of normal sera. In contrast, the absorption at 206 nm of fractions A and B from uremic urine were smaller than that of fractions A and B from normal urine. Fractions A from normal urine, uremic serum and urine were collected and resolved into 3 subpeaks at 254 nm by high performance liquid chromatography. Two of these subpeaks, A-Ⅰ and A-Ⅱ, were detected in uremic serum, normal urine and uremic urine. The results of MALDI-TOF-MS revealed that the fraction A-Ⅰ from both uremic serum and normal urine contained a component with molecular weight 1214, which could hardly be seen in the fraction A-Ⅰ of uremic urine.

Key words: Uremia, Middle molecule, High performance liquid chromatography, Time-of-flight mass spectrometry

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