高等学校化学学报 ›› 2018, Vol. 39 ›› Issue (10): 2178.doi: 10.7503/cjcu20180280

• 分析化学 • 上一篇    下一篇

基于多壁碳纳米管的人参皂苷快速分离

苗瑞1, 吴冬雪1, 王秋颖1, 赵幻希1, 李雪1, 修洋1(), 刘淑莹1,2()   

  1. 1. 长春中医药大学, 吉林省人参科学研究院, 长春130117
    2. 中国科学院长春应用化学研究所, 长春 130022
  • 收稿日期:2018-04-11 出版日期:2018-09-29 发布日期:2018-09-29
  • 作者简介:

    联系人简介: 修 洋, 男, 博士, 助理研究员, 主要从事中药化学方面的研究. E-mail: ys830805@sina.com;刘淑莹, 女, 博士, 教授, 博士生导师, 主要从事有机质谱方面的研究. E-mail: syliu@ciac.ac.cn

  • 基金资助:
    国家自然科学基金(批准号: 21475012)和吉林省科技发展计划项目(批准号: 20160520123JH, 20160309002YY)资助.

Rapid Separation of Ginsenosides Based on Multi-walled Carbon Nanotubes

MIAO Rui1, WU Dongxue1, WANG Qiuying1, ZHAO Huanxi1, LI Xue1, XIU Yang1,*(), LIU Shuying1,2,*()   

  1. 1. Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China
    2. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  • Received:2018-04-11 Online:2018-09-29 Published:2018-09-29
  • Contact: XIU Yang,LIU Shuying E-mail:ys830805@sina.com;syliu@ciac.ac.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.21475012) and the Science and Technology Development Plan Project of Jilin Province, China(Nos.20160520123JH, 20160309002YY).

摘要:

将多壁碳纳米管(MWCNTs)作为选择性吸附材料, 用于快速分离人参提取物中的人参皂苷. 人参经甲醇溶液超声提取后, 提取物中主要为人参皂苷和糖类. 人参中的糖类与人参皂苷的极性相近, 是提取分离人参皂苷时的主要干扰物. MWCNTs可以快速吸附和脱附人参皂苷, 但是对糖类无吸附作用. 利用其选择性吸附性能, 建立了MWCNTs快速分离人参提取物中人参皂苷和糖类的方法. 在优化的分离条件下, MWCNTs对人参提取物中糖类的分离度高于90%, 对8种主要人参皂苷[Rb1, Rb2, Rc, Rd, Re, Rg1, 20(S)-Rf和Ro]的吸附容量为15.0-24.0 μg/mg, 回收率高于90%. 进一步研究表明, 人参皂苷在3 min内即可达到吸附和脱附平衡, 并且人参皂苷的回收率受脱附溶剂极性的影响. 相比于常规材料大孔树脂, MWCNTs可以更快速、 简便地分离人参皂苷.

关键词: 多壁碳纳米管, 人参皂苷, 快速分离, 高效液相色谱-质谱联用

Abstract:

Multi-walled carbon nanotubes(MWCNTs) were employed as the selective adsorbent to investigate their performance in the rapid separation of ginsenoside from ginseng extract. Ginseng was ultrasonically extracted in aqueous methanol. The resulting extract mainly consisted of ginsenoside and saccharide. Saccharide in ginseng was similar with ginsenoside in polarity, and was the main interfering component during the extraction and separation of ginsenoside. Therefore, it is essential to separate saccharide from ginsenoside in ginseng extract. MWCNTs could rapidly adsorb and desorb ginsenoside in ginseng extract, but not the saccharide. Based on this selective adsorption, a novel method using MWCNTs for the rapid separation of ginsenoside and saccharide was developed. Under the optimized separation conditions, MWCNTs had high degree of saccharide separation and ginsenoside recovery, both of which were more than 90%, and also had high adsorption capacity of 15.0—24.0 μg/mg to the 8 main ginsenosides, Rb1, Rb2, Rc, Rd, Re, Rg1, 20(S)-Rf and Ro. Further research revealed that the 8 ginsenosides could reach the adsorptive and the desorptive equilibrium within 3 min, and their recovery were significantly affected by the polarity of desorption solvent. The short equilibrium time was the foundation of the rapid separation of ginsenoside. Comparing with conventional material used for the separation of ginsenoside, such as macroporous resin, MWCNTs-based separation method is less time-consuming, more convenient and efficient.

Key words: Multi-walled carbon nanotubes, Ginsenoside, Rapid separation, High performance liquid chromatography-mass spectrometry(HPLC-MS)

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