高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (4): 663.

• 研究论文 • 上一篇    下一篇

1,4-二氢吡啶衍生物与DNA相互作用的电化学与圆二色谱研究

杜江燕1,2, 王炳祥2, 徐飞2, 陈婷婷2, 沈珠英2, 沈永淼2, 邢巍2, 陆天虹1   

  1. 1. 中国科学院长春应用化学研究所, 长春 130022;
    2. 南京师范大学化学与环境科学学院, 南京 210097
  • 收稿日期:2004-04-28 出版日期:2005-04-10 发布日期:2005-04-10
  • 通讯作者: 陆天虹(1943年出生),男,博士,研究员,博士生导师,从事生物电化学、光谱电化学和化学电源研究.E-mail:tianhonglu@263.net E-mail:tianhonglu@263.net
  • 基金资助:

    江苏省教育厅自然科学基金(批准号:02KJB150006);国家“211”工程重点学科建设项目资助.

Voltammetric and Circular Dichroism Studies on Interaction Between 1, 4-Dihydropyridine Derivatives and DNA

DU Jiang-Yan1,2, WANG Bing-Xiang2, XU Fei2, CHEN Ting-Ting2, SHEN Zhu-Ying2, SHEN Yong-Miao2, XING Wei2, LU Tian-Hong1   

  1. 1. Changchun Institute of Applied Chemistry, Chinese Acdemy of Sciences, Changchun 130022, China;
    2. College of Chemistry and Environment Science, Nanjing Normal University, Nanjing 210097, China
  • Received:2004-04-28 Online:2005-04-10 Published:2005-04-10

摘要: 用电化学和圆二色谱(CD)技术研究了5个1,4-二氢吡啶(1,4-DHP)衍生物与小牛胸腺DNA(CT-DNA)的相互作用.结果表明,1,4-DHP衍生物与CT-DNA相互作用是通过嵌入的方式进行的,相互作用的强弱与1,4-DHP衍生物的立体结构有关.1,4-DHP衍生物4-取代基空间位阻越小,嵌入到CT-DNA的程度越大.相反,空间位阻较大的1,4-DHP衍生物嵌入CT-DNA程度较小.非平面结构的1,4-DHP衍生物使CT-DNA双螺旋链结构松散程度增大.

关键词: 二氢吡啶衍生物, DNA, 电化学, 圆二色谱, 氧化峰, DNA构象

Abstract: The interactions of five kinds of 1,4-dihydropyridine(1,4-DHP) derivatives with calf thymus DNA(CT-DNA) were investigated by the electrochemical method and circular dichroism(CD). The results indicated that the interaction between 1,4-DHP derivatives and CT-DNA was in the intercalative mode. The strength of the interaction increases with the decrease in the steric hindrance of 1,4-DHP derivatives. DNA double helix in solution become more loose in the presence of non-planar structure 1,4-DHP derivatives.

Key words: Dihydropyridine derivatives, DNA, Electrochemistry, Circular dichroism, Oxidation peak, DNA conformation

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