全二维液相色谱(IEC/RP)的构建与评价

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (3): 426.

全二维液相色谱(IEC/RP)的构建与评价

王智聪^1,2, 张庆合^1,2, 张维冰³, 李彤², 赵中一¹

1. 中国地质大学材料与化学工程学院,武汉 430074;
2. 大连依利特分析仪器有限公司,大连 116011;
3. 中国科学院大连化学物理研究所,大连 116011

收稿日期:2004-04-20 出版日期:2005-03-10 发布日期:2005-03-10
通讯作者: 李彤(1962年出生),男,博士,研究员,从事高效液相色谱仪器的研制、生产和应用.E-mail:tonglii@elitehplc.com E-mail:tonglii@elitehplc.com
基金资助:
国家自然科学基金(批准号:20375040)资助

Establishment and Evaluation of Comprehensive Two-dimensional Liquid Chromatography(IEC/RP)

WANG Zhi-Cong^1,2, ZHANG Qing-He^1,2, ZHANG Wei-Bing³, LI Tong², ZHAO Zhong-Yi¹

1. Faculty of Material Science and Chemical Engineering, China University of Geosicences, Wuhan 430074, China;
2. Dalian Elite Analytical Instruments Co., Ltd., Dalian 116011, China;
3. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China

Received:2004-04-20 Online:2005-03-10 Published:2005-03-10

摘要/Abstract

摘要： 以IEC/RP模式构建了二维液相色谱系统,采用平行交替柱捕集分析的2位十通切换阀作接口,第一维洗脱产物按10:1的分流比分流后,得到IEC和RP切换谱图.以5个标准蛋白混合物的分离评价该系统,在单独一维模式中不能分离的样品在全二维液相色谱中得到了很好的分离.

关键词: 全二维液相色谱, 蛋白质, 峰容量

Abstract: A two-dimensional liquid chromatography is established with ion-exchange chromatography(IEC) followed by reversed phase liquid chromatography(RP). The interface is a ten-port two-position switching valve coupled with two parallel RP columns. After splitting the IEC effluent with a ratio of 10:1(volume ratio), the IEC profile and the RP profile were obtained simultaneously. The system is evaluated with 5 standard proteins, which cannot be resolved in either of IEC or RP mode alone but can be resolved by the comprehensive two-dimensional liquid chromatography. The total peak capacity of this system is calculated to be 189.

Key words: Comprehensive two-dimensional liquid chromatography, Protein, Peak capacity

中图分类号:

O658

TrendMD:

王智聪, 张庆合, 张维冰, 李彤, 赵中一. 全二维液相色谱(IEC/RP)的构建与评价. 高等学校化学学报, 2005, 26(3): 426.

WANG Zhi-Cong, ZHANG Qing-He, ZHANG Wei-Bing, LI Tong, ZHAO Zhong-Yi . Establishment and Evaluation of Comprehensive Two-dimensional Liquid Chromatography(IEC/RP). Chem. J. Chinese Universities, 2005, 26(3): 426.

参考文献

[1] Giddings J.C..Ana.lChem.[J],1984,56:1258A—1270A [2] Davis J.M.,Giddings J.C..Ana.lChem.[J],1983,55:418—424 [3] Guiochon G.,Beaver L.A.,Gonnord M.F.et al..J.Chromatogr.[J],1983,255:415—437 [4] Cortes H.J..Multidimensional Chromatography Techniquesand Applications[M],New York:Marcel Dekker,1990 [5] Sheldon E.M..Journalof Pharmaceuticaland Biomedical Analysis[J],2003,31:1153—1166 [6] Szuna A.J.,Blain R.W..J.Chromatogr.[J],1993,620:211—216 [7] Mulligan T.E.,Blain R.W.,Oldfield N.F.et al..J.Liq.Chromatogr.[J],1994,17:133—150 [8] Puhlmann A.,Dulffer T.,Kobold U..J.Chromatogr.[J],1992,581:129—133 [9] Agbaria R.,Ford H.Jr.,Kelley J.A.et al..Ana.lBiochem.[J],1993,213:90—96 [10] Mader R.M.,Rizovski B.,Steger G.G.et al..J.Chromatogr.[J],1993,613:311—316 [11] Sweeney A.P.,Shalliker R.A..Journalof Chromatography A[J],2002,968:41—52 [12] Chen J.Z.,Balgley B.M.,DeVoe D.L.et al..Ana.lChem.[J],2003,75:3145—3152 [13] DavisM.T.,Beierle J.,Bures E.T.et al..Journalof Chromatography B[J],2001,752:281—291 [14] Opiteck G.J.,Lewis K.C.,Jorgenson J.W..Ana.lChem.[J],1997,69:1518—1524 [15] Bushey M.M.,Jorgenson J.W..Ana.lChem.[J],1990,62:161—167 [16] Stroink T.,Wiesea G.,Lingemanb H.et al..Analytica Chimica Acta[J],2001,444:193—203 [17] Unger K.K.,Racaityte K.,Wagner K..J.High Resol.Chromatogr.[J],2000,23:259—265 [18] Wagner K.,Racaityte K.,Unger K.K.et al..Journalof Chromatography A[J],2000,893:293—305 [19] Opiteck G.J.,Ramirez S.M.,Jorgenson J.W..Analytical Biochemistry[J],1998,258:349—361 [20] Wagner K.,Miliotis T.,Marko V.G.et al..Ana.lChem.[J],2002,74:809—820 [21] Schoenmakers P.,Marriott P.,Beens J..L C2GC(Europe)[J],2003,16(6):335—338 [22] ZHAN G Yu-Kui(张玉奎),W AN G Jie(王杰),ZHAN G Wei-Bing(张维冰).Practical H PL C Method Development[M],Beijing:Huawen Press,2002 [23] Moore A.W.,Jorgenson J.W..Ana.lChem.[J],1995,67:3456—3463 [24] Neue U.D.,Mazzeo J.R..J.Sep.Sci.[J],2001,24:921—929