基于再生丝蛋白水凝胶的研究前沿

doi:10.7503/cjcu20170635

摘要/Abstract

摘要：

再生丝蛋白是从蚕丝中得到的纤维状蛋白质. 基于再生丝蛋白的水凝胶在药物控制释放、体外细胞培养、组织工程等多个生物医学领域取得了重要的研究成果, 体现了巨大的应用潜力. 根据不同的物理及化学条件, 再生丝蛋白水凝胶的分子构象可以调节; 除了凝胶浓度、化学交联点密度等因素, 其凝胶性能还可以通过调节β-折叠构象的含量来控制. 本文对再生丝蛋白水凝胶的结构、基本物理化学性质和其在生物医学领域的应用进行综述, 为探索新型生物医用材料提供理论指导.

关键词: 生物高分子, 蛋白质, 支架, 构象, 再生医学, 组织工程, 再生丝蛋白水凝胶

Abstract:

Regenerated silk fibroin(RSF) is a fibrillar protein obtained from Bombyx mori silkworm silk. Recently, RSF-based hydrogels have attracted extensive research interest, which has yielded an improved understanding in the gelling mechanisms, physical and mechanical properties as well as the applications in regenerative medicine and tissue engineering. Mechanistically, RSF hydrogel networks can be formed via two pathways, chemical cross-linking including enzymatic cross-linking, and physical cross-linking through conformational transition, during which thermodynamically more stable β-sheet structures are induced. An advantage of RSF hydrogels is that the physical and mechanical properties of hydrogels can be further controlled by the β-sheet content in the conformation on top of concentration and cross-linking density. RSF can be mixed with other polymers and/or inorganic components to prepare composite hydrogels for a wider spectrum of mechanical properties. Furthermore, we summarize most recent applications of RSF hydrogels in biomaterials, including controlled drug release, muscle, tendon and bone tissue engineering.

Key words: Biopolymer, Protein, Scaffold, Conformation, Regenerative medicine, Tissue engineering, Regenerated silk fibroin hydrogel

中图分类号:

O631

TrendMD:

龙星潼, 管娟, 陈新, 邵正中. 基于再生丝蛋白水凝胶的研究前沿. 高等学校化学学报, 2018, 39(1): 1.

LONG Xingtong, GUAN Juan, CHEN Xin, SHAO Zhengzhong. Progress in Hydrogels Based on Regenerated Silk Fibroin^†. Chem. J. Chinese Universities, 2018, 39(1): 1.

图/表 4

Fig.1 Morphology and network structure of silk-based hydrogels

Fig.2 Ethanol induced RSF hydrogel fibrillar network formation process showing by TEM images at 1 min(A), 10 min(B), 23 min(C) and AFM height images at 10 min(D), 15 min(E) and 23 min(F)Reprinted with permission from Ref.[36], copyright 2010 Royal Society of Chemistry.

Table 1 Mechanical properties of silk hydrogels and silk-based composite hydrogels

Solid content of RSF(%)	Component polymer	Gelation condition	Storage modulus,G'/kPa	Ref.
0.8		pH=2, 50 ℃	3	[28]
3		Sonication	0.01	[45]
4—5		0.5% Ethanol	0.1	[36]
		1% Ethanol	1	[36]
5.4		12% Genipin	46.1	[48]
	80%Gelatin	12% Genipin	1.1	[48]
3	30%PAM	37 ℃	20	[30]
3—4	Collagen	0.05%EDC	3	[49]
	Collagen	0.15%EDC	10	[49]
10	30%HPC	37 ℃	1	[50]
2.4	~40%PEG300	PEG	20	[51]
4.8	~40%PEG300	PEG	54	[51]
5.5	~40%PEG300	PEG	70	[51]
15	~45%PEG300	PEG	70	[51]
15	~42.5%PEG400	PEG	200	[51]

Fig.3 Silk-based hydrogels and their potential applications in tissue engineering

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