在柱透析-毛细管电泳联用分析——一种带有透析采样功能的毛细管电泳的实现与初步应用

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (2): 218.

在柱透析-毛细管电泳联用分析——一种带有透析采样功能的毛细管电泳的实现与初步应用

喻彦林¹, 陈华¹, 肖尚友^1,2, 李鸿义¹, 陶然¹, 夏之宁^1,2

1. 重庆大学化学化工学院药学系;
2. 重庆大学生物力学与组织工程教育部重点实验室,重庆400044

收稿日期:2004-06-01 出版日期:2005-02-10 发布日期:2005-02-10
通讯作者: 夏之宁(1961年出生),男,博士,教授,博士生导师,主要从事分析化学和药物化学研究.E-mail:zhnxia@yahoo.com.cn E-mail:zhnxia@yahoo.com.cn
基金资助:
国家自然科学基金(批准号:20375051);重庆大学应用基础研究基金(批准号:717411019)

A Study of In-line Dialysis Combined with Capillary Electrophoresis——Realization and Preliminary Applications of Capillary Electrophoresis with Dialytic Sampling Function

YU Yan-Lin¹, CHEN Hua¹, XIAO Shang-You^1,2, LI Hong-Yi¹, TAO Ran¹, XIA Zhi-Ning^1,2

1. Department of Pharmaceutics, College of Chemistry and Chemical Engineering;
2. Key Laboratory for Biomechanics & Tissue Engineering of Education Ministry of China, Chongqing University, Chongqing 400044, China

Received:2004-06-01 Online:2005-02-10 Published:2005-02-10

摘要/Abstract

摘要： 在石英毛细管的进样端端口内采用相转移法制作聚砜膜,使该毛细管同时具有采样、样品净化与分离功能.建立了一种透析-毛细管电泳在柱联用方法.膜能有效地拦截大分子和颗粒物,性能稳定,12h内连续使用,膜的透过率和对大分子的拦截性能无显著变化.该方法避免了大分子和颗粒物的干扰,可直接对复杂样品中的小分子进行分析.将其应用于咖啡牛奶中游离咖啡因的含量测定,测得其值为0.68mmol/L;用于药物与蛋白的相互作用研究,获得了盐酸异丙嗪与牛血清白蛋白的结合常数,其值为1.47×10⁴L/mol.

关键词: 透析, 毛细管电泳, 净化采样

Abstract: A novel method of in-line coupling of dialysis with capillary electrophoresis, which eliminates the influence of macromolecules and particles in sample matrixes during the analysis of small molecules, was established in this paper. A polysulfone membrane, which was prepared in the inlet end of the separation capillary by phase-inversion process, cut off macromolecules efficiently and durably, so the capillary can be used for clean-up sampling. Using this capillary in electrophoresis, little column efficiency lost was achieved and the RSD of migration times and peak areas of chlorpheniramine were 0.8% and 4.6%, respectively. During the continuous using of this capillary for 12 h, the performance of the membrane changed little. This technology was applied in the analysis of free caffeine in coffee milk, and with little interference, the concentration was determined to be 0.68 mmol/L. When this method was applied in interaction analysis, the binding constant of promethazine hydrochloride with bovine serum albumin was obtained, that is 1.47×10⁴ L/mol.

Key words: Dialysis, Capillary electrophoresis, Clean-up sampling

中图分类号:

O657

TrendMD:

喻彦林, 陈华, 肖尚友, 李鸿义, 陶然, 夏之宁. 在柱透析-毛细管电泳联用分析——一种带有透析采样功能的毛细管电泳的实现与初步应用. 高等学校化学学报, 2005, 26(2): 218.

YU Yan-Lin, CHEN Hua, XIAO Shang-You, LI Hong-Yi, TAO Ran, XIA Zhi-Ning. A Study of In-line Dialysis Combined with Capillary Electrophoresis——Realization and Preliminary Applications of Capillary Electrophoresis with Dialytic Sampling Function. Chem. J. Chinese Universities, 2005, 26(2): 218.

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