高等学校化学学报

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (S1): 80.

• 研究论文 • 上一篇    下一篇

微流控芯片直接化学发光检测方法研究

吉邢虎, 何治柯, 庞代文, 程介克   

  1. 武汉大学化学与分子科学学院, 武汉 430072
  • 出版日期:2004-12-31 发布日期:2004-12-31
  • 通讯作者: 何治柯
  • 基金资助:

    国家自然科学基金(批准号:20299030-4)资助.

Tris(2,2'-bipyridyl) ruthenium(Ⅱ) Chemiluminescence Reaction and Its Apllication to a Microchip

JI Xing-Hu, HE Zhi-Ke, PANG Dai-Wen, CHENG Jie-Ke   

  1. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China
  • Online:2004-12-31 Published:2004-12-31

PDF (PC)

被引次数

4

摘要：

基于Ru(bipy)32+的化学发光反应,建立了一种新型微流控芯片化学发光检测方法.以草酸为研究对象,探讨了表面活性剂CTAB的浓度、化学发光反应酸度以及Ru(bipy)32+浓度等因素对峰形以及检测灵敏度和重现性的影响.草酸的线性范围为1.0×10-4~5.0×10-6mol/L,检测限达到2.0×10-6mol/L.该方法的建立为微流控芯片分离检测某些有机酸、药物、环境物质及核酸等提供了新方法.

关键词: 微流控芯片, Ru(bipy)32+, 化学发光检测, 草酸

Abstract:

Tris(2,2'-bipyridy) ruthennium(Ⅱ)(Ru(bipy)32+) chemiluminescence(CL) reaction was investigated as a post separation detection scheme for microchip.The oxalic acid was selected as a sample to evaluate the established system.Under the optimum experimental conditions,the linear range and detection limit of oxalic acid were from 5×10-6 to 1×10-4 and 2.0×10-6 mol/L,respectively.The novel microchip CL detection system could be used for the separation and determination of some organic acids and nucleic acids.

Key words: Microchip, Ru(bipy)32+, Chemiluminescence detection, Oxalic acid

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