高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (S1): 128.

• 研究论文 • 上一篇    下一篇

毛细管电泳电致化学发光检测血浆中布比卡因

武亚艳, 李甜, 薛静, 梁恒   

  1. 西安交通大学生物医学信息工程教育部重点实验室, 生命科学与技术学院分离科学研究所, 西安 710049
  • 出版日期:2004-12-31 发布日期:2004-12-31
  • 通讯作者: 梁恒
  • 基金资助:

    国家自然科学基金(批准号:20299030和20175015)资助.

Capillary Electrophoresis Coupled with Tris(2,2'-bipyridyl) Ruthenium Electrochemiluminescence Detection for Bupivacaine Determination

WU Ya-Yan, LI Tian, XUE Jing, LIANG Heng   

  1. The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Separation Science Institute, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China
  • Online:2004-12-31 Published:2004-12-31

摘要:

布比卡因是一种外科局部麻醉剂,使用过量会导致中枢神经系统和心脏血管系统中毒[1],可引起心脏停博.高效液相色谱和毛细管电泳(CE)[2]是该药常用的检测方法.

关键词: 布比卡因, 毛细管电泳, 电致化学发光

Abstract:

The determination of bupivacaine by capillary electrophoresis with Ru(bpy)32+ electroche-miluminescence detection was described.The detection potential,Ru(bpy)32+ concentration,phosphate buffer concentration and pH,injection voltage and time were investigated.Experiment results showed that a good separation for the bupivacaine in plasma can be achieved under the optimized conditions,such as 10 s at 10 kV of electrokinetic injection,10 mmol/L separation phosphate buffer(pH=8.0),15 kV of separation voltage,5 mmol/L Ru(bpy)32+,and 50 mmol/L phosphate buffer(pH 6.5) in the ECL cell.The linear range was 0.02-10μg/mL,the relative standard derivation(RSD) was 3.4%,and the detection limit was 3 ng/mL.When the method was applied to measure bupivacaine in rat plasma,the results were satisfactory.

Key words: Bupivacaine, Capillary electrophoresis, Electrochemiluminesence

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