高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (06): 1162.doi: 10.3969/j.issn.0251-0790.2012.06.008

• 分析化学 • 上一篇    下一篇

基于MCM-41负载联吡啶钌的电致化学发光传感器

王永红1,3,4, 何晓晓1,2,4, 王柯敏1,2,3,4, 苏婧1,2,4, 陈智峰1,2,4, 晏根平1,3,4   

  1. 1. 湖南大学化学生物传感与计量学国家重点实验室, 长沙 410082;
    2. 湖南大学生物学院, 长沙 410082;
    3. 湖南大学化学化工学院, 长沙 410082;
    4. 生物纳米与分子工程湖南省重点实验室, 长沙 410082
  • 收稿日期:2011-11-18 出版日期:2012-06-10 发布日期:2012-06-10
  • 通讯作者: 王柯敏; 何晓晓 E-mail:kmwang@hnu.edu.cn
  • 基金资助:

    国家科技部国际合作专项(批准号: 2010DFB30300)、湖南省自然科学基金创新研究群体(批准号: 10JJ7002)、国家自然科学基金(批准号: 90606003, 20775021, 21190044)和湖南省研究生科研创新项目资助.

Electrogenerated Chemiluminescence Biosensor Based on Ru(bpy)32+ Doped Mesoporous Silica Nanoparticles

WANG Yong-Hong1,3,4, HE Xiao-Xiao1,2,4, WANG Ke-Min1,2,3,4, SU Jing1,2,4, CHEN Zhi-Feng1,2,4, YAN Gen-Ping1,3,4   

  1. 1. State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China;
    2. College of Biology, Hunan University, Changsha 410082, China;
    3. College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China;
    4. Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Hunan University, Changsha 410082, China
  • Received:2011-11-18 Online:2012-06-10 Published:2012-06-10

摘要: 利用静电吸附作用将联吡啶钌[Ru(bpy)32+]负载到巯基化MCM-41介孔二氧化硅纳米颗粒上, 通过金-巯键修饰法将负载后的MCM-41固定在金电极表面, 发展了一种基于MCM-41负载联吡啶钌的电致化学发光传感器, 并研究了其电化学及电致化学发光行为. 基于三聚氰胺与增敏剂三正丙胺氨基结构的相似性, 将负载Ru(bpy)32+的MCM-41电致化学发光传感器用于三聚氰胺的检测, 获得了良好的检测效果, 为检测三聚氰胺提供了一种快速、简便的方法. 同时, 该研究为Ru(bpy)32+在电极表面的固定化提供了新思路.

关键词: 介孔二氧化硅纳米颗粒, 电致化学发光, 联吡啶钌, 三聚氰胺

Abstract: A novel electrogenerated chemiluminescence(ECL) sensor based on Ru(bpy)32+-doped MCM-41 mesoporous silica nanoparticles(MSN) was developed. The Ru(bpy)32+ was first loaded into the sulfhydryl modified MSN through the electrostatic absorption. Then the Ru(bpy)32+-doped MSN was immobilized onto the surface of Au electrode by Au-S interaction. The properties of electrochemistry and electrogenerated chemiluminescence(ECL) were also investigated. The amino structure of melamine is similar to tripropylamine, so we attempted to branch out the application of MCM-41 into the detection of melamine, the ECL sensor provided a new assay for the detection of melamine. Furthermore, the research provided a new approach for the immobilization of Ru(bpy)32+ onto the electrode surface.

Key words: Mesoporous silica nanoparticles, Electrogenerated chemiluminescence, Ru(bpy)32+, Melamine

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