高等学校化学学报

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (7): 1245.

• 研究快报 • 上一篇    下一篇

自旋标记荧光探针表征生物活性分子的自由基损伤

党亚敏, 郭祥群, 赵一兵   

  1. 教育部现代分析科学重点实验室, 化学生物学福建省重点实验室, 厦门大学化学系, 厦门 361005
  • 收稿日期:2004-02-05 出版日期:2004-07-24 发布日期:2004-07-24
  • 通讯作者: 郭祥群(1955年出生),女,博士,教授,从事分子光谱分析研究.E-mail:xqguo@jingxian.xmu.edu.cn E-mail:xqguo@jingxian.xmu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:20375032)资助

Probing Free Radical Damage to Biological Molecules by Spin-labeling Fluorophore

DANG Ya-Min, GUO Xiang-Qun, ZHAO Yi-Bing   

  1. The MOE Key Laboratory of Analytical Science and the Key Laboratory for Chemical Biology of Fujian Province, Department of Chemistry, Xiamen University, Xiamen 361005, China
  • Received:2004-02-05 Online:2004-07-24 Published:2004-07-24

PDF (PC)

被引次数

12

摘要： 生命过程中产生的经基自由基(˙OH)已引起广泛关注.目前,对于˙OH的研究主要集中在直接对˙OH进行定量表征[1~3]和问接检测˙OH诱导损伤生物大分了的损伤产物.˙OH诱导损伤生物大分了,能够产生大量的碳中心自由基[4,5].

关键词: 自旋标记, 荧光探针, 氧化损伤, 碳中心自由基

Abstract: A spin-labeling fluorophore(NA-TEMPO˙), formed by labeling α-naphthalene with a paramagnetic nitroxide(4-hydroxyl-2,2,6,6-tetramethylpiperidinyl-1-oxy), was explored for biologically relevant carbon-centered radicals. The horseradish peroxidase (HPR)/H2O2 reaction was used to generate radicals in derivatives of tyrosine and phenylalanine, and the Fenton reaction was used to generate radicals in deoxyguanosine, guanosine, bovine serum albumin(BSA) and DNA. TEMPO ·-NA, a weak fluorescent compound, showed a dramatic increase in fluorescence intensity after scavenging carbon-centered radicals. The spin-labeling fluorophore with dual function groups, the radical recognizing group and fluorescent group, would be significantly useful in monitoring the formation and translocation of carbon-centered radicals in complex biological systems.

Key words: Spin-labeling, Fluorescent probe, Oxidative damage, Carbon-centered radical

中图分类号: 

TrendMD: