高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (3): 580.

• 研究论文 • 上一篇    下一篇

十八烷基聚氧乙烯表面空间结构和蛋白质吸附行为研究

计剑, 沈家骢   

  1. 浙江大学高分子科学与工程学系, 杭州 310027
  • 收稿日期:2003-02-12 出版日期:2004-03-24 发布日期:2004-03-24
  • 通讯作者: 计 剑(1969年出生),博士,副教授,主要从事生物医用高分子、血液相容性材料、介入医用材料和组织工程材料的研究.E-mail;Jianji@mai.lhz.z.jcn E-mail:Jianji@mai.lhz.z.jcn
  • 基金资助:

    国家自然科学基金(批准号;20174035)资助

Surface Architecture of PMMA-g-SPEO and Its Effects on Protein Adsorption

JI Jian, SHEN Jia-Cong   

  1. Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China
  • Received:2003-02-12 Online:2004-03-24 Published:2004-03-24

摘要: 为探讨聚合物-水界面十八烷基聚氧乙烯链(SPEO)空间结构和白蛋白选择性吸附行为的内在联系,本文采用聚甲基丙烯酸甲酯接枝十八烷基聚氧乙烯(PMMA-g-SPEO),通过不同热处理方式获得了具有“环形链”(A)和“尾形链”(B)结构的两种模型表面.在A表面,水相接触角随水化时间的延长而迅速降低,最终亲水性的界面可同时有效阻抗白蛋白和纤维蛋白原的吸附,但不呈现对白蛋白的选择性吸附;而在B表面,水相接触角随水化时间的延长变化不大,最终疏水性的界面可在有效阻抗纤维蛋白原的吸附同时,有效诱导白蛋白的选择性吸附,具有聚氧乙烯(PEO)阻抗非特异性吸附和十八烷基选择性吸附协同作用的特点.

关键词: 两亲接枝共聚物, 生物仿生, 表面性质, 蛋白质吸附

Abstract: To investigate the effects of SPEO surface architecture onto protein adsorption, a “loop-like” SPEO(surface A) and a “tail-like” SPEO(surface B), which had almost the same surface composition, were constructed Via a special thermal treatment. The contact angle on the Surface A/water interface decreased rapidly with increasing of the water hydration time. After longer water incubation, it presented a hydrophilic interface and impart significant resistant to both albumin and fibrinogen adsorption. But it is non selective for the albumin. By contrast, the contact angle on the Surface B/water interface decreased little with water hydration. It presented a hydrophobic interface, which exhibits a very low fibrinogen adsorption while adsorbing a high level of albumin. The results proved that surface B may keep a “tail-like” SPEO in aqueous solution and it is possible to get a glycocalyx mimic surface, in which a hydrophobic matrix was covered by a layer of flexible hydrophilic PEO and then by hydrophobic signal molecules. The theoretical analysis of the SPEO surface architecture onto protein adsorption was also explored preliminarily.

Key words: Amphiphilic graft copolymer, Biomimetic, Surface properties, Protein adsorption

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