高等学校化学学报

高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (05): 1110.doi: 10.3969/j.issn.0251-0790.2012.05.045

• 高分子化学 • 上一篇    下一篇

胆固醇接枝壳聚糖及其性能

丁珊, 唐敏健, 周长忍, 闵翔, 田金环, 李立华   

  1. 暨南大学材料科学与工程系, 人工器官与材料教育部工程研究中心, 广州 510632
  • 收稿日期:2011-06-15 出版日期:2012-05-10 发布日期:2012-05-10
  • 作者简介:周长忍, 男, 教授, 博士生导师, 主要从事生物医用材料方面的研究. E-mail: tcrz9@jnu.edu.cn
  • 基金资助:

    中央高校基本科研业务费专项资金(批准号: 216113127)、 国家自然科学青年基金(批准号: 30900307)和教育部博士学科点基金(批准号: 200805590001)资助.

Synthesis and Properties of Cholesterol Graft Chitosan

DING Shan, TANG Min-Jian, ZHOU Chang-Ren, MIN Xiang, TIAN Jin-Huan, LI Li-Hua   

  1. Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Department of Material Science and Engineering, Jinan University, Guangzhou 510630, China
  • Received:2011-06-15 Online:2012-05-10 Published:2012-05-10

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被引次数

5

摘要： 以胆固醇琥珀酸单酯对壳聚糖进行仿生功能化改性, 并利用红外光谱和核磁共振波谱对改性后产物进行了表征. 通过接触角测试、 仿生矿化及细胞培养等方法研究了改性后产物的相关性能. 结果表明, 改性后的壳聚糖衍生物接触角有所增大. 在仿生矿化过程中, 随着时间的延长, 壳聚糖衍生物具有对沉积的钙磷盐的稳固作用. 在细胞培养过程中, 改性后的壳聚糖膜上细胞增殖明显. 噻唑蓝(MTT)比色法、 扫描电子显微镜(SEM)与激光共聚焦显微镜(CLSM)表征结果表明, 改性后的壳聚糖膜具有促进3T3细胞黏附、 伸展与增殖的效果.

关键词: 生物仿生, 壳聚糖, 胆固醇, 改性, 仿生矿化, 细胞培养

Abstract: Cholesterol-modified chitosan conjugate with succinyl linkages(CHCS) was synthesized and cha-racterized by Fourier transform infrared(FTIR) and proton nuclear magnetic resonance(1H NMR) spectra. The extent of grafting cholesterol was characterized by elemental analyzer. The properties of CHCS and chitosar(CS) were studied by water contact angle test, biomineralization and cell culture. The results show that the contact angle of CHCS is larger than that of CS. In the process of biomineraliation, CHCS can stable the deposited calcium phosphorus salt. The relationships between CHCS/CS and 3T3 cells were studied by MTT method and cytoske-leton fluorescence staining. The results show that CHCS can promote the cell's adhesion, stretched and proliferation. The introduction of cholesterol can promote the ability of chitosan as biomaterials.

Key words: Bionic, Chitosan, Cholesterol, Modify, Biomineralization, Cell culture

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