高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (10): 1798.

• 研究简报 • 上一篇    下一篇

毛细管电泳电化学发光检测体系中正高压进样条件下正负离子电场放大进样效应

刘继锋, 杨秀荣, 汪尔康   

  1. 中国科学院长春应用化学研究所, 电分析化学国家重点实验室, 长春 130022
  • 收稿日期:2002-12-11 出版日期:2003-10-24 发布日期:2003-10-24
  • 通讯作者: 汪尔康(1933年出生),男,研究员,中国科学院院士,第三世界科学院院士,从事现代电分析化学研究.E-mail:ekwang@ciac.jl.cn E-mail:ekwang@ciac.jl.cn
  • 基金资助:

    国家自然科学基金(批准号:20299030,39990570)资助

Field Amplified Sample Injection of Cationic and Anionic Ions at Positive Voltage in Capillary Electrophoresis with Tris(2,2′-bipyridyl)ruthenium(Ⅱ) Electrochemiluminescence Detection System

LIU Ji-Feng, YANG Xiu-Rong, WANG Er-Kang   

  1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  • Received:2002-12-11 Online:2003-10-24 Published:2003-10-24

关键词: 毛细管电泳, 电化学发光, 电场放大进样

Abstract: The field-amplified sample injection behavior of cationic tripropylamine(TPA) and anionic proline(Pro) at a positive voltage in capillary electrophoresis with tris(2,2′-bipyridyl)ruthenium(Ⅱ) electrochemiluminescence(ECL) detection system was studied.In the case of TPA, where the sample solution was prepared in pure water, ECL sensitivity can be improved by 100 times compared to conventional electroinjection method when a positive voltage was applied.Under the same positive voltage condition, anionic Pro prepared in electrolyte solution can also be injected and concentrated in the column when a water plug was injected before sample introduction.The sensitivity and efficiency were enhanced by 10 and 46 times, respectively.The behavior of cationic TPA can be explained by conventional field amplified sample injection(FASItheory.When the ratio of resistivities of sample matrix to that of separation buffer is less than1(γ<<1), the conventional FASItheory can also be used to explain the improved sensitivity and theoretical plates of Pro.The sensitivity, plate, velocity(vep), amplified factor(vep/vep0) and peak variance(σ2) of Pro reach maximum at optimized water plug length and buffer concentration of the sample matrix.

Key words: Capillary electrophoresis, Electrochemiluminescence, Field-amplified sample injection

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