高等学校化学学报 ›› 2020, Vol. 41 ›› Issue (11): 2421-2425.doi: 10.7503/cjcu20200351

• 庆祝《高等学校化学学报》复刊40周年专栏 • 上一篇    下一篇

电压调制型高灵敏电化学发光分析

张晶晶1,2, 金融2, 方丹君1(), 江德臣2()   

  1. 1.南京医科大学药学院, 南京 211166
    2.南京大学化学化工学院, 生命分析化学国家重点实验室, 南京 210023
  • 收稿日期:2020-06-15 出版日期:2020-11-10 发布日期:2020-11-06
  • 通讯作者: 方丹君,江德臣 E-mail:djf@njmu.edu.cn;dechenjiang@nju.edu.cn
  • 基金资助:
    国家自然科学基金(21974071)

Voltage Modulated Electrochemiluminescence for Highly Sensitive Detection

ZHANG Jingjing1,2, JIN Rong2, FANG Danjun1(), JIANG Dechen2()   

  1. 1.School of Pharmacy,Nanjing Medical University,Nanjing 211166,China
    2.State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210023,China
  • Received:2020-06-15 Online:2020-11-10 Published:2020-11-06
  • Contact: FANG Danjun,JIANG Dechen E-mail:djf@njmu.edu.cn;dechenjiang@nju.edu.cn
  • Supported by:
    Supported by the National Natural Science Foundation of China(21974071)

摘要:

报道了一种电压调制型电化学发光分析技术, 通过在施加恒定电压的基础上叠加一个小脉冲电压, 可将鲁米诺-过氧化氢体系的电化学发光强度提升近2倍. 电化学发光强度与过氧化氢浓度在1.0 nmol/L~200 μmol/L范围内呈现良好的线性关系, 检出限低至0.24 nmol/L(S/N=3). 研究结果表明, 通过电压调制模式可有效提高电化学发光强度, 为深入理解电极界面电化学过程提供了新技术和数据.

关键词: 电化学发光, 电压调制, 鲁米诺, 过氧化氢, 高灵敏检测

Abstract:

A voltage modulated electrochemiluminescent(ECL) method is established for highly sensitive detection. In this new strategy, a small voltage is imposed at a constant voltage that could increase ECL intensity from luminol-hydrogen peroxide by ca. 2 times. This ECL intensity shows a good linear relationship with the concentration of hydrogen peroxide in the range of 1 nmol/L—200 μmol/L with a detection limit of 0.1 nmol/L(S/N=3). The results exhibit that the voltage modulated ECL could be an effective way to increase ECL intensity, and more importantly, used to study the electrochemical process at the electrode interface.

Key words: Electrochemiluminescence, Voltage modulation, Luminol, Hydrogen peroxide, Highly sensitive detection

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