高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (5): 825.

• 研究简报 • 上一篇    下一篇

探针体在蛋白质大分子上Langmuir吸附聚集及应用──蛋白质/荧光桃红B(PB)结合反应研究

郜洪文1, 陈运生2   

  1. 1. 安徽大学化学化工学院, 合肥 230039;
    2. 淮北市环境保护监测站, 淮北 235000
  • 收稿日期:2001-04-25 出版日期:2002-05-24 发布日期:2002-05-24
  • 通讯作者: 郜洪文(1964年出生),男,学士,研究员,从事生物与环境分析化学研究.E-mail:gaohw@mars.ahu.edu.cn E-mail:gaohw@mars.ahu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:40073030);安徽省自然科学基金(批准号:01045301);安徽省优秀青年科技基金资助.

Langmuir Aggregation of Chromophore in Protein Macromoleculeand Its Application──Interaction of Phloxine B with Proteins

GAO Hong-Wen1, CHEN Yun-Sheng2   

  1. 1. College of Chemistry and Chemical Engineering, Anhui University, Hefei 230039, China;
    2. Huaibei Environmental Monitoring Center, Huaibei 235000, China
  • Received:2001-04-25 Online:2002-05-24 Published:2002-05-24

关键词: MASC技术, 蛋白质, Langmur吸附聚集, 荧光桃红B(PB)

Abstract: The microphase adsorption-spectral correction(MASC) technique was applied to investigation ofthe interaction of phloxine B(PB) with proteins.The aggregation of PBobeys the Langmuir monolayeradsorption and it has been used in the determination of protein solution at pH3.8.The results show thatthe adsorption ratios of PBto proteins are n(PB)∶ n(BSA) = 1:155, n(PB):n(OVA) = 1:58, n(PB):n(Mb) = 1:18, their adsorption constants are 2.17 × 104, 1.18 × 104, 3.25× 105 and their real molarabsorptivities εBSA-PB555nm=7.94 × 106, εOVA-PB555nm=3.39× 106, εMb-PB555nm= 1.21 × 106 L ·mol-1 ·cm-1.The interac-tion of PBwith proteins is sensitive and selective in the presence of EDTA.For analysis of samples, the re-covery of protein is between 96.7% and 107% and the relative standard deviation 4.6%.

Key words: MASC technique, Protein, Langmuir aggregation, Phloxine B(PB)

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