高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (8): 1349.

• 研究简报 • 上一篇    下一篇

丝组二肽对牛血清白蛋白的切割作用研究

陈晶, 万荣, 刘海, 蒋宇扬, 赵玉芬   

  1. 清华大学生命科学与工程研究院化学系, 生命有机磷化学教育部重点实验室, 北京 100084
  • 收稿日期:2000-06-08 出版日期:2001-08-24 发布日期:2001-08-24
  • 通讯作者: 赵玉芬(1948年出生),女,博士,教授,博士生导师,中国科学院院士,主要从事生命有机化学研究.
  • 基金资助:

    国家自然科学重点项目基金(批准号:29632004);访问学者基金(批准号:教技司[1999]153号)

Studies on the Cleavage of Bovine Serum Albumin by Ser-His

CHEN Jing, WAN Rong, LIU Hai, JIANG Yu-Yang, ZHAO Yu-Fen   

  1. The Key Laboratory of Bioorganic Phosphorus Chemistry, Ministry of Education, Department of Chemistry, School of Life Sciences and Engineering, Tsinghua University, Beijing 100084, China
  • Received:2000-06-08 Online:2001-08-24 Published:2001-08-24

关键词: 丝组二肽, 牛血清白蛋白, 蛋白切割

Abstract: It has been found that Ser-His could cleave bovine serum albumin(BSA), with the most suitable pHaround 6.0. The kinds of buffers have different influences on the cleavage activity. The phosphate buffer and BRbuffer are the most effective. The hydroxyl group of the serine residue is the essential functional group for the cleavage, while the amide bond, imidazole, and carboxyl group of Ser-His increase the cleavage activity.

Key words: Seryl histidine dipeptide, Bovine serum albumin(BSA), Protein cleavage

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