高等学校化学学报

高等学校化学学报 ›› 2013, Vol. 34 ›› Issue (2): 375.doi: 10.7503/cjcu20120645

• 有机化学 • 上一篇    下一篇

光谱法研究硫鸟嘌呤与七元瓜环及牛血清白蛋白的超分子相互作用

黄英1, 王娟2, 郭改英2, 陶朱2, 薛赛凤2, 祝黔江2, 周清娣3   

  1. 1. 贵州大学西南药用生物资源教育部工程研究中心;
    2. 贵州省大环化学及超分子化学重点实验室, 贵阳 550025;
    3. 悉尼大学化学院, 澳大利亚新南威尔士州 2006
  • 收稿日期:2012-07-06 出版日期:2013-02-10 发布日期:2013-01-21
  • 通讯作者: 陶朱,男,博士,教授,博士生导师,主要从事超分子化学研究.E-mail:gzutao@263.net;周清娣,女,博士,教授,主要从事超分子化学研究.E-mail:q.zhou@chem.usyd.edu.au E-mail:gzutao@263.net;q.zhou@chem.usyd.edu.au
  • 基金资助:

    国家自然科学基金(批准号: 20972034)、 贵州省教育厅自然科学基金(批准号: 黔教科2010013号)、 贵州省科技厅国际合作项目(批准号: 黔科合外G字[2012]7003号)和教育部春晖计划项目(批准号: Z2011034)资助.

Interaction of 6-Thioguanine with Cucurbit[7]uril and Bovine Serum Albumin by Spectroscopic Method

HUANG Ying1, WANG Juan2, GUO Gai-Ying2, TAO Zhu2, XUE Sai-Feng2, ZHU Qian-Jiang2, ZHOU Qing-Di3   

  1. 1. The Engineering and Research Center for Southwest Bio-Pharmaceutical Resources, Ministry of Education;
    2. Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province, Guizhou University, Guiyang 550025, China;
    3. School of Chemistry, the University of Sydney, NSW 2006, Australia
  • Received:2012-07-06 Online:2013-02-10 Published:2013-01-21

PDF (PC)

被引次数

46

摘要：

利用紫外-可见吸收光谱法和荧光光谱法研究了抗癌药物硫鸟嘌呤(6-TG)与七元瓜环(Q[7])及牛血清白蛋白(BSA)的相互作用. 结果表明, 6-TG与Q[7]及BSA可形成三元复合物, 且6-TG与Q[7]及BSA均可形成1:1的超分子配合物, 6-TG能引起BSA的荧光猝灭, 猝灭机制为静态猝灭. 此外, 还用同步荧光法和三维荧光法考察了6-TG对BSA构象的影响, 结果表明6-TG的加入使BSA的构象发生了变化, 而同步荧光光谱结果表明结合位点更接近于色氨酸.

关键词: 硫鸟嘌呤, 七元瓜环, 牛血清白蛋白, 紫外-可见吸收光谱, 荧光光谱

Abstract:

The aim of the present study was to investigate the interaction of 6-thioguanine(6-TG) with cucurbit[7]uril(Q[7]) and bovine serum albumin(BSA) in an acetate buffer solution by ultraviolet absorption spectroscopy, fluorescence emission spectroscopy and synchronous fluorescence spectra, as well as and three-dimensional fluorescence spectra. It was indicated from UV-Vis spectra that 6-TG-Q[7] binary complex formed. The fluorescence quenching of BSA by 6-TG was a result of the formation of 6-TG-BSA complex, and the quenching mechanism of BSA by 6-TG was static quenching mechanism. Moreover, effect of 6-TG on BSA conformation was discussed by the studies of synchronous and three-dimensional fluorescence spectra. In addition, a specific binding interaction took place between the 6-TG and Q[7] in the presence of BSA.

Key words: 6-Thioguanine, Cucurbit[7]uril, Bovine serum albumin, Ultraviolet absorption spectroscopy, Fluorescence spectroscopy

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