高等学校化学学报 ›› 2013, Vol. 34 ›› Issue (8): 1818-1825.doi: 10.7503/cjcu20130098

• 研究论文: 无机化学 • 上一篇    下一篇

邻菲啰啉和氨三乙酸钴配合物与牛血清白蛋白相互作用的荧光分析

林海彬1, 郑琳1, 林玉琴1, 周朝晖2   

  1. 1. 漳州师范学院化学与环境科学系, 福建省现代分离分析科学与技术重点实验室, 漳州 363000;
    2. 厦门大学固体表面物理化学国家重点实验室, 厦门 361005
  • 收稿日期:2013-01-28 出版日期:2013-08-10 发布日期:2013-07-19
  • 通讯作者: 林海彬,男,讲师,主要从事生物无机化学方面的研究.E-mail:linhb_97@fjzs.edu.cn E-mail:linhb_97@fjzs.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 21073150)资助.

Study of Interaction Between Bovine Serum Albumin and Cobalt Complexes of Phenanthroline and Nitrilotriacetate by Fluorescence Spectrometry

LIN Hai-Bin1, ZHENG Lin1, LIN Yu-Qin1, ZHOU Zhao-Hui2   

  1. 1. Department of Chemistry and Environmental Science, Fujian Province University Key Laboratory of Analytical Science, Zhangzhou Teachers College, Zhangzhou 363000, China;
    2. State Key Laboratory for Physical Chemistry of Solid Surface, Xiamen University, Xiamen 361005, China
  • Received:2013-01-28 Online:2013-08-10 Published:2013-07-19

摘要:

利用溶液法合成了邻菲啰啉(phen)和氨三乙酸(H3nta)钴配合物Co(phen)2Cl2(1), Na[Co(nta)]·H2O(2), [Co(phen)2(H2O)2][Co(nta)(phen)]2·12H2O(3), 对配合物3进行了X射线单晶衍射表征, 结果表明: 该配合物属三斜晶系, P1空间群, a=1.2448(2) nm, b=1.5898(3) nm, c=1.7412(3) nm, α=91.746(3)°, β=97.807(3)°, γ=103.745(3)°, V=3.309(1) nm3. 利用荧光光谱法研究了室温下这3种配合物与牛血清白蛋白(BSA)的相互作用, 并测定了不同温度下这3种配合物与BSA相互作用的荧光强度变化, 确定配合物13对BSA的荧光猝灭方式均为静态猝灭; 分析了配合物13与BSA相互作用时的结合常数、 结合位点数以及热力学函数与温度之间的关系, 进一步讨论了这2种配合物分别与BSA相互作用时的作用位点、 作用力的类型以及两者之间的距离.

关键词: 钴离子, 邻菲啰啉, 氨三乙酸, 牛血清白蛋白, 荧光光谱

Abstract:

Cobalt complexes [Co(phen)2Cl2(1), Na[Co(nta)]·H2O(2), [Co(phen)2(H2O)2]·[Co(nta)(phen)]2·12H2O([STHZ]3[STBZ])] (phen: phenanthroline, nta: nitrilotriacetate) were synthesized in solution, and the structure of complex 3 was characterized by single-crystal X-ray diffraction analysis(XRD). It crystallized in the triclinic system, space group P1 with a=1.2448(2) nm, b=1.5898(3) nm, c=1.7412(3) nm, α=91.746(3)°, β=97.807(3)°, γ=103.745(3)°, V=3.309(1) nm3. The interaction between bovine serum albumin(BSA) and complexes 1-3 was investigated with fluorescence spectra at room temperature. Fluorescence quenchings were shown as static quenching for complexes 1 and 3 based on the fluorescence spectrum at different temperatures. The binding constant, binding sites and thermodynamic functions were calculated. The interaction sites, binding force and distance for BSA with complexes 1 and 3 were discussed respectively.

Key words: Cobalt(Ⅱ), Phenanthroline, Nitrilotriacetic acid, Bovine serum albumin(BSA), Fluorescence spectrum

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