高等学校化学学报

高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (4): 598.

• 研究简报 • 上一篇    下一篇

丝组二肽对DNA的切割作用的研究

万荣, 王宁, 赵玉芬   

  1. 清华大学生命科学与工程研究院化学系, 教育部生命有机磷化学重点实验室, 北京 100084
  • 收稿日期:2000-03-06 出版日期:2001-04-24 发布日期:2001-04-24
  • 通讯作者: 赵玉芬(1948年出生),女,博士,教授,博士生导师,中国科学院院士,主要从事生命有机磷化学的研究.
  • 基金资助:

    国家自然科学基金(批准号:29632004)资助

Studies on DNA Cleaved by Seryl-histidine Dipeptide

WAN Rong, WANG Ning, ZHAO Yu-Fen   

  1. Key Laboratory of Bioorganic Phosphorus Chemistry of Education Ministry, Department of Chemistry, School of Life Science and Engineering, Tsinghua University, Beijing 100084, China
  • Received:2000-03-06 Online:2001-04-24 Published:2001-04-24

PDF (PC)

被引次数

38

关键词: 丝组二肽, DNA, 切割, 水解机理

Abstract: Linear and supercoiled DNAwere cleaved by HPLCpurified seryl-histidine dipeptide(SH). It was found that the DNAfragments produced by the reaction of SHand DNAcould be ligated together by T4 DNAligase. This result implied that the SHwas the first example of the ion free artificial DNAcleavage agent that could split DNAby hydrolysis mechanism.

Key words: Seryl-histidine dipeptide, DNA, Cleavage, Hydrolysis mechanism

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