高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (4): 538.

• 研究简报 • 上一篇    下一篇

白眉蝮蛇蛇毒及蛇毒酶的激光解吸飞行时间质谱研究

孙明忠, 刘志强, 丁兰, 赵大庆, 刘淑莹, 倪嘉缵   

  1. 中国科学院长春应用化学研究所, 长春 130022
  • 收稿日期:1999-06-24 出版日期:2000-04-24 发布日期:2000-04-24
  • 通讯作者: 赵大庆(1963年出生),男,博士,研究员,主要从事生物无机化学研究.
  • 基金资助:

    吉林省科学技术委员会基金(批准号:962907)

Mass Spectrometry Study on Snake Venoms and Enzymes

SUN Ming-Zhong, LIU Zhi-Qiang, DING Lan, ZHAO Da-Qing, LIU Shu-Ying, NI Jia-Zuan   

  1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  • Received:1999-06-24 Online:2000-04-24 Published:2000-04-24

关键词: 蛇毒, 酶, 激光解吸飞行时间质谱

Abstract: In this paper, three kinds of snake venoms and four kinds of enzymes (phospholipase A2, fibrinolytic enzyme, arginine esterase and L-amino acid oxidase) isolated from the snake venom were analyzed. As the snake venom was different, the MALDI/TOF/MSshowed difference. The MALDI/TOF/MSdetermination results could be affected by the concentrations of snake venom enzymes. And the mechanisms of desorption and ionization was also given in this study. By using MALDI/TOF/MSwe obtained the accurate molecular weights and homogeneities of the enzymes. The apparent characteristics of the positive MALDI/TOF/MSof enzymes composed by two subunits were also given out. The results showed that MALDI/TOF/MSis an effective analytic method for discovering new components from snake venom complexes. And it is reliable to use this method to determine the molecular weights and purifies of protein molecules.

Key words: Snake venoms, Enzymes, MALDI/TOF/MS

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