高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (2): 198.

• 论文 • 上一篇    下一篇

KIO3存在下溶菌酶的极谱催化波研究与应用

过玮, 杨亚妮, 宋俊峰   

  1. 西北大学电分析化学研究所, 西安 710069
  • 收稿日期:1999-04-23 出版日期:2000-02-24 发布日期:2000-02-24
  • 通讯作者: 宋俊峰(1944年出生),男,教授,博士生导师,主要从事电分析化学研究.
  • 基金资助:

    国家自然科学基金(批准号:29875017);陕西省自然科学基金(批准号:97H07)资助

Studies and Application of Polarographic Catalytic Wave of Lysozyme in the Presence of KIO3

GUO Wei, YANG Yia-Ni, SONG Jun-Feng   

  1. Institute of Electroanalytical Chemistry, Northwest University, Xi'an 710069, China
  • Received:1999-04-23 Online:2000-02-24 Published:2000-02-24

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摘要： 研究了溶菌酶(Lysozyme,LE)在KIO3存在下的极谱催化波.在0.1mol/LHAc-NaAc(pH4.7±0.1)缓冲液中,LE有1个由Cys6～Cys127间双硫键还原产生的可逆波.其峰电位为-0.51V(vs.Ag/AgCl).当KIO3存在时,在原电位处产生了LE的极谱催化波.该催化波是由于KIO3及其中间价态衍生质点(包括自由基IO-·,IO2-·)氧化LE还原产物巯基成双硫键所致.这是蛋白质极谱催化波的一种新类型.在0.1mol/LHAc-NaAc(pH4.7±0.1)-1×10-3mol/LKIO3支持电解质中,LE催化波的灵敏度比其还原波的高两个数量级,峰电流与LE浓度在2×10-7～1.0×10-6mol/L范围内有线性关系.100倍半胱氨酸和胱氨酸不干扰1.0×10-6mol/LLE的测定.

关键词: 蛋白质, 溶菌酶, KIO3, 极谱催化波

Abstract: Apolarographic catalytic wave of lysozyme(LE) in the presence of KIO3is described. In 0.1 mol/L HAc-NaAc(pH4.7±0.1) buffer solution, LEyields a reversible wave with peak potential -0.51 V (vs. Ag/AgCl) due to the reduction of a disulfide bond between cys6-cys127. When KIO3is present in the acetate solution, LEproduced a polarographic catalytic wave at original potential. The mechanism of the catalytic wave was considered that the disulfide bond was regenerated through oxidation reaction of reduction product -SHgroup by KIO3and its derived species with intermediate valence state(including free radical IO-·, IO2-·). This is a new class of polarographic catalytic wave for protein. In01mol/L HAc-NaAc(pH4.7±0.1)-1×10-3mol/L KIO3supporting electrolyte, the sensitivity of the catalytic wave is two orders of magnitude higher than that of the corresponding reduction wave. The peak current of the catalytic wave was linearly proportional to LEconcentration in the range 2×10-7-1×10-6mol/L. 100-fold cystine and cystein do not interfere the determination of 1×10-6mol/L LE.

Key words: Protein, Lysozyme, KIO3, Polarographic catalytic wave

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