高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (1): 82.

• 研究快报 • 上一篇    下一篇

金属硫蛋白溶液聚合状态的研究

侯廷军1, 安钰2, 毕汝昌3, 茹炳根2, 徐筱杰1   

  1. 1. 北京大学化学与分子工程学院;
    2. 北京大学生命科学学院, 北京 100871;
    3. 中国科学院生物物理研究所, 北京 100101
  • 收稿日期:1999-06-29 出版日期:2000-01-24 发布日期:2000-01-24
  • 通讯作者: 徐筱杰(1937年出生),男,教授,博士导师,主要从事计算化学研究.
  • 基金资助:

    国家自然科学基金重大项目(批准号:29992590)资助.

Studies on the Metallothionein Polymerization in Different Buffers

HOU Ting-Jun1, AN Yu2, BI Ru-Chang3, RU Bing-Gen2, XU Xiao-Jie1   

  1. 1. College of Chemistry and Molecular Engineering, Peking University;
    2. College of Life Sciences, Peking University, Beijing 100871, China;
    3. Instituteof Biophysics, Chinese Academyof Sciences, Beijing 100101, China
  • Received:1999-06-29 Online:2000-01-24 Published:2000-01-24

关键词: 金属硫蛋白, 聚合, 分子识别

Abstract: In this paper, the naturally occurred polymerization for rabbit metallothionein monomers has been studied in different milieu, while leaves out the aggregation caused by the oxidation of cysteine, since the latter circumstance was the result of purification lapsus. After the purification of metallothionein monomers, dynamic light scattering technique is used to detect the polymerized states of rabbit liver metallothionein Ⅰ and Ⅱ in different buffers, which is the first systematical detection of polymerized states of MTs in solutions. The effects of different factors contributing to methothionein aggregations have been investigated by computational simulations. The calculation results showed that the metallothionein polymerization would be regulated by many factors including electrostatic interactions, hydrophobic interactions and different ions in buffers. A molecular recognition mechanism of MTs' polymerization in solutions is proposed on the bases of experimental results and theoretical calculations.

Key words: Metallothionein, Polymerization, Molecular recognition

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