高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (4): 523.

• 研究快报 • 上一篇    下一篇

Δ-和Λ-[Ru(bpy)2(HPIP)]2+两种异构体对小牛胸腺DNA不同键合速率的CD光谱证明

刘劲刚1, 叶保辉1, 计亮年1, 田暄2, 傅瑶红3   

  1. 1. 中山大学化学系, 广州 510275;
    2. 兰州大学应用有机化学国家重点实验室, 兰州 730000;
    3. 上海有机化学研究所生命有机与天然产物国家重点实验室, 上海 200032
  • 收稿日期:1999-02-01 出版日期:1999-04-24 发布日期:1999-04-24
  • 通讯作者: 计亮年
  • 作者简介:刘劲刚,男,27岁,博士研究生.
  • 基金资助:

    国家自然科学基金(批准号:29731031);广东省基金(970211)资助课题

Observation of the Different Binding Rates of Δ-and Λ-[Ru(bpy)2(HPIP)]2+with Calf Thymus DNA via CD Spectra

LIU Jin-Gang1, YE Bao-Hui1, JI Liang-Nian1, TIAN Xuan2, FU Yao-Hong3   

  1. 1. Department of Chemistry, Zhongshan University, Guangzhou, 510275;
    2. State Key Laboratory, of Applied Organic Chemistry, Lanzhou University, Lanzhou, 730000;
    3. State Key LaboLaboratory of Bio-Organic and Natural Products, Shang hai Institute of Organic Chemistry, Shanghai, 200032
  • Received:1999-02-01 Online:1999-04-24 Published:1999-04-24

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摘要： Wilson于1997年发表了关于DNA导电性的评论性文章,介绍了各种学派的观点.争论焦点在于DNA是否为导体.将两种配合物键合于DNA作为接头,通过研究电子转移可确定DNA是否为导线,其中配合物和DNA键合模式是研究的关键.

关键词: 钌配合物, DNA, 手性异构体, CD光谱, 键合速率

Abstract: The complexes of Δ-and Λ-[Ru(bpy)2(HPIP)]2+(bpy=2,2'-bipyridine, HPIP=2-(2-hydroxyphenyl)imidazo [4,5-f][1,10]phenanthroline) were prepared and their racemic complex were dialysed against calf thymus DNA(CT-DNA). During the course of dialysis with the solution stirring for 48h, the CD signals of the dialyzate experience disappearing-appearing-disappearing. However, in the similar conditions except for not stirring, strong CD signals appeared. From the results obtained, several novel opinions were suggested. Firstly, the evidence of different binding rates of Δ-andΛ-enantiomers of polypyridyl ruthenium(Ⅱ) complexes with CT-DNA, the first example, was demonstrated. As for the dialysis experiment, binding of -enantiomer is dynamically preferred for CT-DNA. Secondly, the different binding rates may be deduced from different binding modes of the enantiomers.

Key words: Ruthenium(Ⅱ) complexes, DNA, Enantiomers, CD spectra, Binding rate

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