高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (12): 1868.

• 研究快报 • 上一篇    下一篇

鲨鱼软骨血管抑制因子的高效液相色谱与电喷雾质谱研究

岳贵花1, 杨芃原1, 沈先荣2, 吉冬梅2, 任大明2, 刘为平2, 许云敏2, 王洪海2   

  1. 1. 复旦大学化学系, 上海 200433;
    2. 复旦大学遗传所, 上海 200433
  • 收稿日期:1999-08-24 出版日期:1999-12-24 发布日期:1999-12-24
  • 通讯作者: 杨芃原
  • 作者简介:岳贵花,女,27岁,博士研究生.
  • 基金资助:

    国家教育部科技重点项目基金;博士点基金(批准号:98024614);复旦大学资助课题

Identification of Angiogenesis Inhibitory Factor by HPLC and ESIMS

YUE Gui-Hua1, YANG Peng-Yuan1, SHEN Xian-Rong2, JI Dong-Mei2, REN Da-Ming2, LIU Wei-Ping2, XU Yun-Min2, WANG Hong-Hai2   

  1. 1. Department of Chemistry, Fudan University, Shanghai 200433, China;
    2. Institute of Genetic, Fudan University, Shanghai 200433, China
  • Received:1999-08-24 Online:1999-12-24 Published:1999-12-24

PDF (PC)

被引次数

6

关键词: 高效液相色谱, 电喷雾质谱, 鲨鱼软骨血管抑制因子

Abstract: The studies of SCAIF-I(an unknown protein) by HPLC and ESIMS was reported in this paper. The SCAIF Iis a kind of antiangiogenesis extracted from shark cartilage. The extracted protein was purified by HPLC and then studied by ESIMS. The molecule weight of the SCAIF-I protein was confirmed to be 15601 in the first time. Peptide map of the unknown protein was obtained by HPLC-ESIMS with a trypsin digestion and possible partial sequences were deduced with the online CIDtechnique. With partial sequence information the homologous proteins are searched with protein database. The homology of SCAIF-I summarized in this work is usefuLIn further studies of this unknown protein.

Key words: HPLC, ESIMS, SCAIF

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