高等学校化学学报

高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (3): 353.

• 论文 • 上一篇    下一篇

毛细管电泳质谱联用技术测定肽和蛋白质混合物

周国华1, 罗国安1, 周克玉2, 古卓良3   

  1. 1. 清华大学生命科学与工程研究院, 北京, 100084;
    2. 中国科学院南京土壤研究所, 南京, 210018;
    3. 南京军区后勤部药品检验所, 南京, 210002
  • 收稿日期:1997-02-06 出版日期:1998-03-24 发布日期:1998-03-24
  • 通讯作者: 罗国安
  • 作者简介:周国华,男,34岁,博士研究生.
  • 基金资助:

    国家自然科学重点基金(批准号:296350220)资助课题.

On-line Capillary Electrophoresis-mass Spectrometry for Determination of Peptide Mixture and Protein Mixture

ZHOU Guo-Hua1, LUO Guo-An1, ZHOU Ke-Yu2, GU Zhuo-Liang3   

  1. 1. School of Life Science and Engineering, Tsinghua University, Beijing,100084;
    2. Institute of Soil Science, Chinese Academy of Sciences, Nanjing,210018;
    3. Nanjing Military Institute for Drug Control, Nanjing, 210002
  • Received:1997-02-06 Online:1998-03-24 Published:1998-03-24

PDF (PC)

被引次数

27

摘要： 以血管紧张素Ⅰ、Ⅱ、Ⅲ、P-物质和生长激素抑制剂混合物为测定对象,研究了未涂层柱、线性聚丙烯酰胺(LPA)涂层柱和胺涂层柱对毛细管电泳分离肽类混合物的影响.结果表明:适合质谱测定的缓冲液不能在未涂层柱上有效地分离5种肽类混合物,而pH为5.O和4.5的NH4Ac缓冲液却能在LPA涂层柱和胺涂层柱上很好地分离上述物质.用CE-ESI-MS法分离鉴定了5种生物活性肽和蛋白质混合物中的肌红蛋白、碳酸酐酶Ⅱ,实测了促红细胞生成素(EPC))的胰酶消解肽图,并对CE-MS联用测定中的影响因素进行了讨论.

关键词: 毛细管电泳-质谱联用技术, 涂层毛细管柱, 肽, 蛋白质, EPO胰酶肽图

Abstract: The influence of uncoated, coated linear polyacrylamide(LPA) and amino coated capillaries on the separation of five peptide mixtures, including angiotensin Ⅰ, Ⅱ, Ⅲ, substance P and somatostain, was studied. The results showed that the peptide mixtures could not be separated completely in uncoated capillary by volatile buffer which was suitable for the MSanalysis, whereas they could be separated in LPA and amino coated capillary by pH 5.0 and pH 4.5 NH4Ac buffer respectively. In addition, the above peptide mixtures, protein mixtures comprised of myoglobin and carbonic anhydrase Ⅱ, tryptic digests of erythropoietin, were separated and characterized by capillary electrophoresis mass spectrometry(CE-ESI-MS) with electrospray ionization interface. The factors affecting the determination in CE-ESI-MS were also discussed.

Key words: Capillary electrophoresis-mass spectrometry, Coated capillary, Peptide, Protein, Tryptic mapping of erythropoietin

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