高等学校化学学报 ›› 1997, Vol. 18 ›› Issue (9): 1459.

• 研究简报 • 上一篇    下一篇

葡萄糖氧化酶光敏氧化反应过程的毛细管电泳法监测

胡平, 罗国安, 王如骥, 董润安, 宋心琦   

  1. 清华大学化学系, 北京 100084
  • 收稿日期:1996-07-18 出版日期:1997-09-24 发布日期:1997-09-24
  • 通讯作者: 罗国安.
  • 作者简介:胡平, 女, 24岁, 硕士研究生.
  • 基金资助:

    国家自然科学基金

Studies on the interaction of Photosensitizers and Glucose Oxidase Using Capillary Electrophoresis

HU Ping, LUO Guo-An, WANG Ru-Ji, DONG Run-An, SONG Xin-Qi   

  1. Department of Chemistry, Tsinghua University, Beijing 100084
  • Received:1996-07-18 Online:1997-09-24 Published:1997-09-24

关键词: 毛细管电泳, 葡萄糖氧化酶, 光敏氧化

Abstract: The interaction of glucose oxidase (GOD) and four photosensitizers, ZnpcS4,ZnPc(NHCH2COOH)4, CIAIPcS, and porphyrine-like, were monitored by capillaryelectrophoresis. GOD, its degenerated products and photosensitizers were simultaneouslyseparated in a buffer of 300 mmol/Lborate at PH8.5 on a commercial CEinstrument. Bycomparison of the electropherograms of GODas well as the mixture of GODand photosensitizerswithout/with irradiation of different time, it was found that photosensitized oxidationsbetween GODand different photosensitizers have different reaction mechanisms and kinetics.

Key words: Capillary electrophoresis, Glucose oxidase, Photosensitized oxidation

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