高等学校化学学报 ›› 1997, Vol. 18 ›› Issue (7): 1046.

• 综合评述 • 上一篇    下一篇

微柱分离用于单个细胞分析

程介克, 王宗礼   

  1. 武汉大学化学系, 武汉 430072
  • 收稿日期:1996-08-10 出版日期:1997-07-24 发布日期:1997-07-24
  • 通讯作者: 程介克, 男, 65岁, 教授, 博士导师.
  • 作者简介:程介克, 男, 65岁, 教授, 博士导师.
  • 基金资助:

    国家自然科学基金

Analysis of Single Cells by Microcolumn Separation

CHENG Jie-Ke, WANG Zong-Li   

  1. Department of Chemistry, Wuhan University, Wuhan 430072
  • Received:1996-08-10 Online:1997-07-24 Published:1997-07-24

摘要: 对90年代发展的微柱分离用于单个细胞水平的分析化学进行了评述. 系统地论述了单个细胞取样方法学,毛细管电泳及微柱液相色谱分离、电化学、激光诱导荧光、免疫、放射性检测方法及其应用于单个细胞分析的新进展.

关键词: 单个细胞分析, 微柱分离, 毛细管电泳, 开管液相色谱, 高效液相色谱

Abstract: The application of microcolumn separation developed in 1990s to analytical chem-istry at the single cell level is reviewed.The new developments of single cell samplingmethodology, capillary electrophoresis and microcolumn liquid chromatographic separationand detection methods (electrochemistry, laser-induced fluorescence, immunology and ra-diometry) and their application to analysis of single cells are described in detail.

Key words: Single cell analysis, Microcolumn separation, Capillary electrophoresis, Open tubular liquid chromatography, High performance liquid chromatography

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