高等学校化学学报

高等学校化学学报 ›› 1997, Vol. 18 ›› Issue (7): 1035.

• 论文 • 上一篇    下一篇

过氧化物酶新型荧光底物3,4-二氢-2(1H)-喹喔啉酮的反应机理及性能的研究

高军, 李元宗, 黄兰, 吴新元, 慈云祥   

  1. 北京大学化学与分子工程学院, 北京 100871
  • 收稿日期:1997-03-04 出版日期:1997-07-24 发布日期:1997-07-24
  • 通讯作者: 李元宗
  • 作者简介:高军, 女, 29岁, 博士研究生.
  • 基金资助:

    国家自然科学基金

Studies on the Reaction Mechanism and Properties of a New Peroxidase Fluorogenic Substrate Dihydro-2 (1H) -quinoxalone

GAO Jun, LI Yuan-Zong, HUANG Lan, WU Xin-Yuan, CI Yun-Xiang   

  1. Institute of Chemistry and Molecular Engineering, Peking University, Beijing 100871
  • Received:1997-03-04 Online:1997-07-24 Published:1997-07-24

PDF (PC)

被引次数

4

摘要： 合成了过氧化物酶新型荧光底物3,4-二氢-2(1H)-喹喔啉酮(DHQXL). 在过氧化物酶催化下,该化合物可被H2O2氧化生成强荧光物质2(1H)－喹喔啉酮(QXL). 用高压液相色谱法分离得荧光产物纯品,经核磁共振谱和质谱证实其结构,确证了提出的酶催化反应机理. 对该化合物作为荧光底物的性能研究表明,它是一个很有应用前景的过氧化物酶的新型荧光底物.

关键词: 荧光底物, 3, 4-二氢-2(1H)-喹喔啉酮, 辣根过氧化物酶, 模拟酶

Abstract: Anew fluorogenic substrate, 3, 4-dihydro-2 (1H)-quinoxaloe (DHQXL), for per-oxidase was synthesized- It can be converted to strong fluorescent compound 2(1H)-quinox-alone(QXL) in the presence of horseradish peroxidase (HRP) or its mimetic enzymes heminand Mn-TPPS4. Pure fluorescent product was isolated by HPLC.The structure of the prod-uct was determined using1H NMRand MSspectroscopic techniques, which confirmed theproposed reaction mechanism for the enzyme catalyzed reaction.The property of the sub-strate was evaluated- The experimental results showed that it is a fluorogenic substrate forperoxidase that has a high potential in future application.

Key words: Fluorogenic substrate, 3,4-Dihydro-2 (1H)-quinoxalone, Horseradish peroxi-dase, Mimetic enzyme

中图分类号: 

TrendMD: