高等学校化学学报 ›› 1996, Vol. 17 ›› Issue (9): 1345.

• 论文 • 上一篇    下一篇

荧光法研究手性金属配合物与DNA的作用机理

靳兰1, 杨频2, 李青山1   

  1. 1. 南京大学配位化学研究所, 南京, 210093;
    2. 山西大学分子科学研究所, 太原, 030006
  • 收稿日期:1995-09-11 出版日期:1996-09-24 发布日期:1996-09-24
  • 通讯作者: 杨频
  • 作者简介:靳兰,女,28岁,博士研究生.
  • 基金资助:

    国家自然科学基金

Studies on the Mechanism of the Chiral Metal Complexes with DNA by Fluorimetry Method

JIN Lan1, YANG Pin2, LI Qing-Shan1   

  1. 1. Coordination Chemistry Institute of Nanjing University, Nanjing, 210093;
    2. Inslitute of Molecular Science, Shanxi University, Taiyuan, 030006
  • Received:1995-09-11 Online:1996-09-24 Published:1996-09-24

摘要: 以溴化乙锭为荧光探针,研究手性金属配合物[Ni(phen)3]2+和[Fe(phen)3]2-与DNA的反应机理,结果表明,配合物与DNA作用存在插入和静电结合两种模式,即部分菲咯啉配体插入DNA双螺旋碱基对中,同时带正电荷的配合物与DNA的磷酸基团发生静电结合。

关键词: 手性金属配合物, DNA, EB探针

Abstract: The fluorescence characteristics of EBwhich is sensitive to DNAwas used as a probe to study the interaction of [Ni(phen)3]2+,[Fe(phen)3]2+ with DNA.The results show that the complexes bind to DNAby two models, one is the partial insertion of one of the ligands between the base pairs on the DNAduplex strand, the other is electrostatic binding by which the complexes can interact electrostatically with the phosphate backbone of DNA.

Key words: Chiral metal complexes, DNA, EB probe

TrendMD: