高等学校化学学报

高等学校化学学报 ›› 1996, Vol. 17 ›› Issue (5): 829.

• 研究简报 • 上一篇    

SDS-PAGE研究丝素蛋白的组成

于同隐1, 蔡再生1, 黄伟达2   

  1. 1. 复旦大学高分子科学研究所, 上海 200433;
    2. 复旦大学生物化学系, 上海 200433
  • 收稿日期:1995-05-30 出版日期:1996-05-24 发布日期:1996-05-24
  • 通讯作者: 于同隐,男,79岁,博士导师,教授.
  • 作者简介:于同隐,男,79岁,博士导师,教授.
  • 基金资助:

    国家自然科学基金;国家博士后基金;上海市博士后基金

Investigation of Fibroin Composition of Bombyx Mori Silk Proteins by SDS-PAGE

YU Tong-Yin1, CAI Zai-Sheng1, HUANG Wei-Da2   

  1. 1. Institute of Macromolecular Science, Fudan University, Shanghai 200433;
    2. Department of Biochemistry, Fudan University, Shanghai 200433
  • Received:1995-05-30 Online:1996-05-24 Published:1996-05-24

PDF (PC)

被引次数

22

关键词: 丝素蛋白, 亚基组成, 聚丙烯酰胺凝胶, 电泳

Abstract: The fibroin proteins directly extracted from silk glands of Bombyx mori were used for polyacrylamide gel electrophoresis in th presence of sodium dodecyl sulfate (SDS-PAGE).The subunit structure of fibroin molecule was discussed.It has been elucidated that the native fibroin molecule consists of subunits a,b and c with molecular weights of 280 kD, 230 kD and 25 kD, respectively, Since the amount of them is much more than that of the b subunit, the a and b subunits are the major component of fibroin.Of which the b subunit is composed of smaller e subunit of Mw about 130 kDand f subunit of MW about 125 kD,linked by disulfide bonds.

Key words: Fibroin protein, Subunit structure, Polyacrylamide gel, Electrophoresis

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