关键词: 血清白蛋白, 偶氮胂Ⅲ, 染色反应

Abstract: The interaction of arsenazo Ⅲ(AAⅢ) with bovine serum albumin(BSA) in acidicsolution was investigated by a spectrophotometric method.It was considered that the electrostatic force between AAⅢ and BSAis the main binding force,and that the color changeduring the reaction is because of the transformation of AAⅢspecies from free H₆L^2- form into bound H₅L^3- form.The apparent binding constant and the maximum binding number under the conditions of PH2.0, with 0.5% NaCl and without NaCl, were estimated respectively by using a linear regression method proposed previously by the authors.The sensitivity ofdetection for BSAwas found to be about the same as that of the bromocresol green method.

Key words: Serum albumin, Arsenazo Ⅲ, Staining reaction