高等学校化学学报

高等学校化学学报 ›› 1995, Vol. 16 ›› Issue (2): 201.

• 论文 • 上一篇    下一篇

疏水界面上标准蛋白质吸附等温线的研究

边六交, 耿信笃   

  1. 西北大学现代分离科学研究所, 西安 710069
  • 收稿日期:1994-02-01 修回日期:1994-11-02 出版日期:1995-02-24 发布日期:1995-02-24
  • 通讯作者: 耿信笃
  • 作者简介:边六交,男,29岁,助研.
  • 基金资助:

    “863”生物领域资助

Studies on Adsorption Isotherms of Proteins on Hydrophobic Surface

BIANL Liu-Jiao, GENG Xin-Du   

  1. Institute of Modern Separation Science, Northwest University, Xi'an, 710069
  • Received:1994-02-01 Revised:1994-11-02 Online:1995-02-24 Published:1995-02-24

PDF (PC)

被引次数

13

摘要： 测定了几种具有代表性的标准蛋白质在疏水色谱填料上的吸附等温线.除溶菌酶和牛血清白蛋白的吸附近似呈线性外,细胞色素-c、肌红蛋白、胰岛素、α-淀粉酶和卵清蛋白均呈凸型吸附.用Langmuir、计量置换吸附模型(SDM-A)、BET和Jovanovic吸附模型对这些蛋白的吸附等温线拟合后发现,SDM-A关系式能够良好描述这7种蛋白的吸附,Langmuir关系仅能较好地描述后5种蛋白质的吸附.而Jovanovic和BET关系式对上述两类蛋白质吸附的描述欠妥.实验发现对于不同盐浓度条件下卵清蛋白的吸附,用SDM-A和Langmuir公式拟合所得的参数与盐浓度之间呈现出一定的规律性.

关键词: 吸附等温线, 蛋白质, 疏水色谱

Abstract: Adsorption isotherms of proteins on hydrophobic surface were measured by frontal chromatography.It was found the adsorption isotherms of lysozyme and bovine serum albumin to be linear and those of cytochrome-c,myoglobin,insulin,α-amylase,and ovalbumin to be convex.With the comparison among stoichiometric displacement model of adsorption(SDM-A),the Langmuir model,the BET equation,and the Jovanovic equation,the first one was found to fit two kinds of adsorption isotherms mentioned above well,the second one could only be used to describe the latter well,while the last two equations could not be used to describe anyone of them well.For ovalbumin,the linear parameters of the SDM-A and the Langmuir model were found to have certain regularity with the changes in the concentrations of ammonium sulfacte.

Key words: Adsorption isotherms, Proteins, Hydrophobic interaction chromatography

TrendMD: