高等学校化学学报

高等学校化学学报 ›› 1995, Vol. 16 ›› Issue (12): 1907.

• 论文 • 上一篇    下一篇

Tb3+,Ca2+与天花粉蛋白的结合效应

刘国红1, 李文连3, 郝强1, 赵旭3, 张岩1, 杨红1, 黄仲立2, 高贵2, 董庆初1, 李青山1   

  1. 1. 吉林大学分子生物学系, 长春, 130023;
    2. 吉林大学酶工程国家重点实验室, 长春, 130023;
    3. 中国科学院长春物理研究所
  • 收稿日期:1994-12-30 修回日期:1995-08-21 出版日期:1995-12-24 发布日期:1995-12-24
  • 通讯作者: 李青山
  • 作者简介:刘国红,女,23岁,硕士研究生.
  • 基金资助:

    国家自然科学基金;吉林大学酶工程国家重点实验室和吉林大学分子光谱和分子结构开放实验室资助课题

Binding Effect of Tb3+and Ca2+Ions on Trichosanthin

LIU Guo-Hong1, LI Wen-Lian3, HAO Qian1, ZHAO Xu3, ZHANG Yan1, YANG Hong1, HUANG Zhong-Li2, GAO Gui2, DONG Qing-Chu1, LI Qing-Shan1   

  1. 1. Department of Molecular Biology , Jilin University, Changchun, 130023;
    2. National Laboratory of Enzyme Engineering, Jilin University, Changchun, 130023;
    3. Changchun Institute of Physics, Chinese Academy of Sciences
  • Received:1994-12-30 Revised:1995-08-21 Online:1995-12-24 Published:1995-12-24

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摘要： 利用Tb3+作为荧光探针研究天花粉蛋白(TCS)的结构。Tb3+可置换天花粉蛋白中的Ca2+,发生从TCS分子中的Trp残基到Tb3+的能量传递,使Trp的荧光发射强度减弱。利用光谱技术研究发现,Tb3+与TCS亲和力小于Ca2+与TCS的亲和力;Tb3+与其结合后基本不改变TCS的构象,但Trp和Tyr残基处的微环境发生变比;Ca2+对TCS构象有显著影响。

关键词: Tb3+, Ca2+, 天花粉蛋白, 荧光探针

Abstract: Tb3+has been used as a fluorescent probe to study the structure of trichosanthin(TCS).The nonradioactive energy transfer from fluorescent active tryptophan(Trp)residue to the bound Tb3+ions take place and the fluorescence of Trp is decreased when Tb3+ions are bound to TCSto replace the Ca2+in it.According to the results of Trp fluorescence and the information given from CDand UVabsorption spectra,we have found that the affinity of Tb3+to TCS is lower than that of Ca2+ions to TCS. Significant change of TCS structure was not observed with the Tb3+ions binding,The microenvironment surrounded Trp and Tyr may be changed slightly with the Tb3+binding.The conformation of Ca2+-free TCS was significantly changed compared with that of native TCS with the Trp residue moving into the interior of TCS molecules。 The Ca2+-binding site in TCS can be maintained partly when the Ca2+is removed from TCSmolecules.

Key words: Tb3+,Ca2+, Trichosanthin, Fluorescent probe

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