高等学校化学学报

高等学校化学学报 ›› 1995, Vol. 16 ›› Issue (12): 1856.

• 论文 • 上一篇    下一篇

镀铂玻碳电极电聚3,3′-二氨基联苯二胺固定葡萄糖氧化酶传感器的研究

张占恩, 邓家祺   

  1. 复旦大学化学系, 上海, 200433
  • 收稿日期:1994-12-27 修回日期:1995-04-09 出版日期:1995-12-24 发布日期:1995-12-24
  • 通讯作者: 邓家棋
  • 作者简介:张占恩,男,32岁,讲师,博士研究生.
  • 基金资助:

    国家自然科学基金;中国科学院长春应用化学研究所电分析化学开放实验室基金

A Study of Glucose Biosensor Based on Immobilization of Glucose Oxidase in Electrochemically Polymerized Poly(3, 3′-diaminobenzidine) Film on Platinized Glassy Carbon Electrode

ZHANG Zhan-En, DENG Jia-Qi   

  1. Department of Chemistry, Fudan University, Shanghai, 200433
  • Received:1994-12-27 Revised:1995-04-09 Online:1995-12-24 Published:1995-12-24

PDF (PC)

摘要： 用镀铂玻碳电极电化学聚合3,3′-二氨基联苯二胺固定葡萄糖氧化酶(GOD)制成电流型葡萄糖传感器。镀铂电极铂黑沉积层的微孔结构使电极具有较大的有效表面积,GOD固定于铂黑微粒孔隙中具有较高的稳定性。传感器具有响应快、灵敏度高、线性范围宽、稳定性好等优点,并具有抗抗坏血酸、抗尿酸干扰的特点。

关键词: 生物传感器, 3, 3&prime, -二氨基联苯二胺, 电化学聚合, 葡萄糖氧化酶, 镀铂玻碳电极

Abstract: An amperometric glucose biosensor has been developed based on immobilization of GODin electrochemically synthesized poly (3, 3′-diaminobenzidine) (PDAB) film on a platinized glassy carbon electrode(PGCE).The PGCE provides a large apparent surface area ,and GOD entrapped in the of the electrodeposited platinum particles is more stable than on a platinum disc electrode surface. The sensor respeonses to β-D-glucose linearly over a concentration range of 1×10-6to 1×10-3mol/L, and the detection limit is 5. 1×10-7mol/L.At a concentration of 0. 1 mmol/Lglucose, the response current of the sensor is fifty fold higher than that of the sensor prepared with a platinum disc electrode.The response time of the sensor is less than 4 s and the life time is over 6 months.Due to the permselective characteristics of the PDAB film, the effects of electroactive interferents such as ascorbic acid and uric acid, can be significantly reduced.

Key words: Biosensor, 3,3′-Diaminobenzidine, Electropolymerization, Glucose oxidase.Platinized glassy carbon electrode

TrendMD: