高等学校化学学报 ›› 1994, Vol. 15 ›› Issue (2): 189.

• 论文 • 上一篇    下一篇

聚邻苯二胺修饰电极抗坏血酸氧化酶生物传感器的研究

金利通, 赵桂珠, 方禹之   

  1. 华东师范大学化学系, 上海, 200062
  • 收稿日期:1993-04-10 修回日期:1993-10-15 出版日期:1994-02-24 发布日期:1994-02-24
  • 通讯作者: 金利通,男,56岁,教授.
  • 作者简介:金利通,男,56岁,教授.
  • 基金资助:

    上海市科委科学基金

A Study of Ascorbic Acid Sensor Based on Ascorbic Oxidase Immobilized in an Electropolymerized Poly(o-phenylenediamine) Film

JIN Li-Tong, ZHAO Gui-Zhu, FANG Yu-Zhi   

  1. Department of Chemistry, East China Normal University, Shanghai, 200062
  • Received:1993-04-10 Revised:1993-10-15 Online:1994-02-24 Published:1994-02-24

摘要: 本文报道了聚邻苯二胺/抗坏血酸氧化酶生物传感器,用这种传感器测定人体血清中的抗坏血酸,线性范围在1.0×10-4~2.5×10-7mol/L之间,响应时间为7s,检测限为1.0×10-8mol/L.该传感器具有选择性好、灵敏度高和响应时间短等特点。

关键词: 生物传感器, 抗坏血酸氧化酶, 酶电极, 邻苯二胺, 修饰电极

Abstract: A chemically modified electrode biosensor is reported.The biosensor has been applied to determine ascorbic acid in human serum.The linear range of detection is 1.0×10-5-2.5×10-7mol/L and detection limit is 1.0×10-8mol/L.The response time is 7 s.The biosensor has advantages of high selectivity,sensitivity and short response time.

Key words: Biosensor, Ascorbate oxidase, Enzyme electrode, o-Phenylenediamine, Modified electrode

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