高等学校化学学报

高等学校化学学报 ›› 1991, Vol. 12 ›› Issue (7): 939.

• 论文 • 上一篇    下一篇

荧光探针Tb(Ⅲ)与葡萄糖淀粉酶的络合作用

翟国堂1, 袁静明1, 袁滔2   

  1. 1. 山西大学分子科学研究所, 太原, 030006;
    2. 华东化工学院生化工程研究所
  • 收稿日期:1990-02-23 出版日期:1991-07-24 发布日期:1991-07-24
  • 通讯作者: 袁静明
  • 基金资助:

    国家自然科学基金

The Complexing of Glucoamylase with Fluorescent Probe Tb(Ⅲ)

Zhai Guo-tang1, Yuan Jing-ming1, Yuan Tao2   

  1. 1. Institute of Molecular Science, Shanxi University, Taiyuan, 030006;
    2. Institute of Biochemical Engineering, East China University of Chemical Technology, Shanghai
  • Received:1990-02-23 Online:1991-07-24 Published:1991-07-24

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摘要： 以Tb(Ⅲ)作荧光探针探索葡萄糖淀粉酶分子与该离子的成键位置数、成键环境及键合Tb(Ⅲ)的解离常数KD。

关键词: Tb(Ⅲ), 荧光探针, 葡萄糖淀粉酶, 成键性质, 络合作用

Abstract: The bonding properties, enviroment and the dissociation constant of glucoamylase with Tb(Ⅲ) as a fluorescent probe were investigated. The results show that an enzyme molecule could be bonded with 4 Tb(Ⅲ) at 20℃> pH 4.6, and the bonding site is in the free carboxyl groups which belong to Glu/Asp, KD was calculated to be 2. 5×10-4mol/L. The fluorescence intensity of Tb(Ⅲ) is greatly sensitized by the enzyme to 1000 folds at 545 nm. On the contrary, the fluorescence intensity of glucoamylase at 345 nm is quenched and the quenching efficiency is calculated to be 0. 75%.

Key words: Tb(Ⅲ), Fluorescence probe, Glucoamylase, Bonding property, Complexing

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